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Appendix I: Microscope settings

40

Useful

magnification

You can determine the total magnification by dividing the length of the object
shown by the set length on the micrometer. The useful magnification of the
optical systems microscope depends on the numerical aperture of the objective
being used (engraved on the objective). The useful magnification is in the range
of 500x to 1,000x the numerical aperture.

Empty

magnification

A loss of contrast and sharpness is the result if total magnification is larger than
the useful magnification. Check to see if replacing the TV adapter with a 0.5 or
0.63 model sufficiently reduces the total magnification. If this is not possible, you
can also use an objective with the same magnification, but with a higher
numerical aperture.

Содержание SC30

Страница 1: ...User Manual SC30 3 MegaPixel CMOS Color Camera for Light Microscopy...

Страница 2: ...fy the purchaser In no event shall Olympus Soft Imaging Solutions GmbH be liable for any indirect special incidental or consequential damages arising out of purchase or use of this manual or the infor...

Страница 3: ...ion 25 Additional commands 27 Open 27 Print Directly 29 Print Setup 30 Print 31 File list 32 Exit 33 Scale Bar Show in Viewport 33 Scale Bar Burn into Image 33 Delete Image 34 Image Manager 34 Status...

Страница 4: ...Contents 4 Appendix III In case of problems with the camera 59 Appendix IV WEEE declaration 61 Appendix V EMC declaration SC30 62 Appendix VI RoHS declaration SC30 63 Index 64...

Страница 5: ...ading 1 ms exposure time Technical data SC30 Chip Type CMOS Chip Size Inch 1 2 Effective Area H x V mm x mm 6 55 x 4 92 Pixel Size m x m 3 2 x 3 2 Binning Color binning 2x 3x 4x Resolutions H x V at f...

Страница 6: ...ecommendations can t include all of the systems resp hardware combi nations that are possible Hardware Software Processor Intel Pentium D better than or equal to 3 0 GHz Intel Xeon DP MP Intel Core Du...

Страница 7: ...start the setup exe file via Windows Explorer with a doubleclick 3 An installation wizard guides you through the entire software installation Simply follow the onscreen instructions and select the re...

Страница 8: ...hannels in such a way that this requirement is satisfied in the best possible way for the area selected White balance is carried out on a part of the image ROI Your software alters the color settings...

Страница 9: ...adaptation of the color temperature is not part of the operating system but rather is made accessible by the monitor manufacturer or the graphics card being used 5 Select a color temperature whose co...

Страница 10: ...ur loaded images It is possible to view up to 9 images simultaneously To display them the image window is divided up into several windows i e viewports Each viewport can display a single image Images...

Страница 11: ...The GUI User Interface of getIT 11...

Страница 12: ...t viewport Image buffers will be reassigned Zoom factors will be set to Auto Though reduced in size somewhat the entire test image will be shown 3 Click the Single View button to display just one imag...

Страница 13: ...on programs which have access to a TWAIN interface Examples for this are MS Word and Adobe Photoshop Note If you are using an imaging system solution provided by Olympus instead of the image acquisiti...

Страница 14: ...s only necessary when several cam eras are available and the wrong camera has been selected Your image acquisition software enables you to manage several cameras Each camera is assigned to a predefine...

Страница 15: ...Channel button if you have deleted an input channel by mistake or if the camera driver has not been integrated successfully into the software Click the Delete Channel to delete a camera s input channe...

Страница 16: ...nd focus on the object as well as the possibility to optimally set your camera s settings Camera The Camera field shows you the selected cameras with their serial numbers Resolution The Resolution lis...

Страница 17: ...ve increased the lamp voltage you should carry out another white balance Click the and buttons to change the exposure time in pseudo logarithmic intervals You can also click the arrow buttons next to...

Страница 18: ...tness Use the Brightness slide control to change the image s intensity Values smaller than 1 lower the image s intensity Values greater than 1 increase the image s intensity Contrast Use the Contrast...

Страница 19: ...mage information as well This means that when you are planning on quantitatively evaluating your images later you should not use the sharpen filter Your software provides you with commands with which...

Страница 20: ...tion the width and height of an object area is assigned to the pixel representing that object area This might be for example 0 63 m x 0 63 m for a magnification of 5x This calibration data var ies for...

Страница 21: ...s 30 and 80 in the example shown is 500 m 8 Select the Arbitrary option in the Calibration group 9 Now click the Calibrate button The mouse pointer will appear within the active image 10 Position the...

Страница 22: ...ta use the Apply calibration button You can view the calibration data of an image any time by double click ing the image buffer to open the Image Information dialog box Description of the dialog box N...

Страница 23: ...o create the calibration data as described starting on page 20 1 Acquire an image or click on the image buffer in which the image to be calibrated has been stored 2 Click the Apply calibration button...

Страница 24: ...e Save in list File list The dialog box shows all the files with the file extension shown in the Save as type field Files of type The Save as type list contains all the file formats under which the ac...

Страница 25: ...image Except for the name of the image this image data are only then saved when you save the image in TIF format Alternative Use the Alt Enter shortcut to view data about the image in the active image...

Страница 26: ...ame of the camera for images acquired with your camera The field does not contain any information for other images Magnification The Magnification field shows the selected magnification for images whi...

Страница 27: ...sed on standard MS Windows dialog boxes The dialog box for loading images also has a preview function Look in Select the drive and directory of the desired files from the Look in list File list The di...

Страница 28: ...ffer box click the image buffer you wish to load the image into with the left mouse button Select for example image buffer 5 The selected image buffer will be highlighted in color 2 Click the Open but...

Страница 29: ...e loaded 4 Repeat the last two steps if you wish to load further images 5 To quit loading just click the Close button Use the mouse to drag drop images into the image buffer desired MS Explorer a file...

Страница 30: ...quisi tion software from the printer list Status Type Where Comment The Status Type Where and Comment fields provide information about the selected printer operating state printer type path name and a...

Страница 31: ...t Setup dialog box is preset The printer selected for the previous prints will be offered for each additional print Status Type Where Comment The Status Type Where and Comment fields provide informati...

Страница 32: ...ffer box Range of images Option Select the Range of images option to print a range of images from the image buffer box To do so you enter the number of the image buffers whose images you would like to...

Страница 33: ...y adjusted to fit the cur rent zoom factor of the image in the viewport You may also have the unit auto matically adjusted in order to avoid excessively large or small numeric values You can alternati...

Страница 34: ...Deleting selected images To delete multiple images all at once select them in the image buffer box Press Shift or Ctrl while selecting the images with the mouse Now the command in the Image menu will...

Страница 35: ...log box with the same name What s it for The dialog box contains various lists with information pertaining to your system image acquisition software and hardware components such as your camera It also...

Страница 36: ...More Info button to open the System information Windows dialog box This enables you to take a look at the assembly and configuration of your com puter as well as your operating system s settings and c...

Страница 37: ...minator This illustration clarifies the basic assembly of the components of a microscope in transmitted light mode Illumination Light is a decisive medium for creating images with a microscope As a re...

Страница 38: ...advantage ND filter Many microscopes can insert ND filters into the light path thus reducing the intensity of the lamp s light These ND filters ensure a uniform reduction of light intensity throughout...

Страница 39: ...de 40 0 65 marks an objective with a magnification of 40x and the numerical aperture of 0 65 Magnification An objective s engraved magnification is considerably important for the total magnification H...

Страница 40: ...ive being used engraved on the objective The useful magnification is in the range of 500x to 1 000x the numerical aperture Empty magnification A loss of contrast and sharpness is the result if total m...

Страница 41: ...gently with an air blower For dust stuck due to static electricity ionized air is recommended Warning It is best to use a smooth brush to clean the cover slip Do this only when absolutely necessary W...

Страница 42: ...mera onto your microscope 3 Connect the camera to your PC and select the camera driver USB 2 0 interface If you want to use the camera you will need to have a USB 2 0 interface available on your PC If...

Страница 43: ...s built in on a separate board no guarantee can be made that the camera can be used without any problems The Device Manager dialog box provides no information as to whether the interface is available...

Страница 44: ...ras SC20 SC30 XM10 XC10 UC30 XC30 UC50 XC50 Installing the image acquisition software getIT 1 Place the CD that has been supplied into the CD ROM drive The installation program will start automaticall...

Страница 45: ...Driver dialog box opens 4 Select the TWAIN driver for your camera Please note a special driver is required for the SC20 and the SC30 cameras Select the SC20 resp SC30 check box Select the Olympus Sof...

Страница 46: ...ry 7 Click the Next button The Choose Folder dialog box opens 8 Select the installation folder You can either browse to an existing folder or create a new one 9 Click the Next button The getIT softwar...

Страница 47: ...n order to read the getIT software manual Select the No do not install it option for instance if the Acrobat Reader program is already installed 11 To start the actual installation click the Next butt...

Страница 48: ...ding on its surface 3 Install this assembly onto the microscope s camera mount Some micro scopes may require additional optical elements installed between the camera mount and the C mount adapter You...

Страница 49: ...driver After connecting the camera to your PC the Found New Hardware Wizard will automatically start Registering the camera driver The camera driver is automatically installed when setting up your ima...

Страница 50: ...and select the Install the software automatically Recommended option and click the Next button The installation begins After a little while the Hardware Installation dialog box will pop up 4 Click the...

Страница 51: ...n In doing so the registration of your camera driver with Windows XP is not yet finished Windows XP opens the Found New Hardware Wizard dialog box a second time 6 Repeat steps 2 to 5 In doing so the r...

Страница 52: ...ied into the CD ROM drive The installation program will start automatically unless you have deactivated the autorun function If so please start the setup exe file manually 2 If Microsoft Windows Vista...

Страница 53: ...confirm them with OK The installation of the getIT image acquisition software will be prepared The following dialog box opens 5 Select the language of your getIT software The default language is your...

Страница 54: ...Select the Olympus Soft Imaging Solutions Firewire Cameras check box with all the other cameras XM10 XC10 UC30 XC30 UC50 XC50 The Choose Destination Location dialog box opens 7 If you want to change t...

Страница 55: ...hether the Acrobat Reader program should be installed automatically together with the getIT image acquisition software 11 Select the Yes install it option if you want to automatically install the Acro...

Страница 56: ...ion software has now been installed Mounting the camera The diagram shows a typical installation on a light microscope You should have all of the required components ready at hand Warning Install the...

Страница 57: ...he C mount adapter You may also need to align the orientation of the microscope with that of the stage and also match the focus of the camera image with that of the eyepiece image These settings can b...

Страница 58: ...Vista will then ask to be allowed to activate the device driver 2 Confirm the message by clicking the Continue button 3 Wait until Microsoft Windows Vista has opened the displayed security query 4 Cli...

Страница 59: ...provided please feel free to contact our customer service preferably via e mail Our customer service personnel will be more than happy to assist you 1 Please clarify before hand when and under what c...

Страница 60: ...o our customer service address support olympus sis com The quickest and most convenient way is to use the automatic e mail settings for your customer service query Via the About System Info Send butto...

Страница 61: ...lectronic Equipment In accordance with European Directive 2002 96 EC on Waste Electrical and Electronic Equipment this symbol indicates that the product must not be disposed of as unsorted municipal w...

Страница 62: ...Appendix V EMC declaration SC30 62 Appendix V EMC declaration SC30...

Страница 63: ...Appendix VI RoHS declaration SC30 63 Appendix VI RoHS declaration SC30...

Страница 64: ...fer 10 Image buffer box 10 Hide 34 Image display Optimize 12 Single view Tile view 12 Image information 25 Image name 25 26 Image window 10 Input Set 14 Installation of software and camera driver 7 K...

Страница 65: ...ort 33 Scan 19 Serial Number 60 Sharpen filter 18 Special 9 System Info 35 60 System requirements 6 T Total magnification microscope 8 TWAIN dialog box 16 TWAIN interface 13 U Useful magnification Mic...

Страница 66: ...OLYMPUS SOFT IMAGING SOLUTIONS CORP 200 Union Blvd Suite 202 Lakewood CO 80228 U S A Phone 1 303 234 9270 Fax 1 303 234 9271 info osis olympus sis com OLYMPUS SOFT IMAGING SOLUTIONS PTE LTD No 2 Juron...

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