Olympus SC30, Руководство пользователя, Страница: 17 / 66

Acquiring and saving images
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The exposure time required for high-quality acquisitions depends on the illumi-
nation, the properties of the sample, and the camera being used. The exposure
time should be longer than 1 ms and shorter than 125 ms. Should the exposure
time be outside of these limits, you can take the following actions.
Exposure time shorter
than 1 ms
The best means of increasing the exposure time is by placing neutral density
filters in your microscope's light path. If no neutral density filters are available,
you should reduce your lamp voltage. But make sure that the voltage of a
halogen lamp doesn't drop to below 5 V. When you have reduced your lamp
voltage you will need to carry out a white balance again.
Exposure time longer
than 125 ms
To reduce the exposure time, first remove any ND-filters that are in use. On the
microscope, you can also additionally set the prism or the phototube's mirror to
"only photo". Should the result still not be adequate, you can then increase the
lamp voltage. After you have increased the lamp voltage, you should carry out
another white balance.
Click the [-] and [+] buttons to change the exposure time in pseudo-logarithmic
intervals. You can also click the arrow buttons next to the display field to alter the
exposure time in linear intervals.
Click the Auto button to automatically set the exposure time.
White Balance
Click the White Balance button to correct a tinge. During a white balance, the
individual color channels for red, green, and blue are set in such a way, that a
white or gray area of an object will be portrayed correctly in white, i.e., gray. All
of the other colors will be shown correctly, as well.
A red rectangle appears in the image. Move it with the mouse to a position as
uniformly white or gray as possible. Change the size of the rectangle by moving
the mouse and simultaneously depressing the mouse button. Rightclick to adopt
position and size of the rectangle and execute the white balance.
Color Settings
Click the Color Settings button to manually alter your camera's color settings.
Based on the type of camera you are using, this may be done via numerous slide
controls.
This is what the Color
Settings dialog box
looks like for the SC30
camera.
Related Topics
White Balance 8