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11
TRANSMITTED LIGHT BRIGHTFIELD
OBSERVATION PROCEDURE
}The following flow shows the operating procedure for the transmitted light brightfield observation which is the basic obser-
vation method of this microscope. The operating procedures for phase contrast observation and DIC observation will be
described separately in Chapter 5, “OTHER OBSERVATION METHODS” on page 28.
For the fluorescence observation, refer to the separate instruction manual entitled “Reflected Fluorescence System”.
(Controls Used)
(Page)
Start observation.
Set the main switch to “
I
” (ON).
1
Main switch: “
I
” (ON)
(P. 13)
2
Transmitted light ON-OFF button: ON
(P. 13)
Disengage the DIC slider and
analyzer from the light path.
Disengage the filter other than the
frost filter from the light path.
3
Filter pocket
(P. 21)
Engage a 10X objective in the light path.
7
Objective switching button
(P. 14)
Select the brightfield observation by pressing
the BF button
*
and select the (observation)
light path.
4
BF observation button
*
5
Light path selector button
(P. 13)
Place the specimen on the stage.
6
X-axis and Y-axis knobs
(P. 17)
Bring the specimen in focus.
8
Focusing knob (dial)
(P. 15)
Focus adjustment knob or
focusing button
Adjust the brightness.
9
Light intensity control buttons
(P. 13)
Adjust the interpupillary distance.
Adjust the diopter.
Adjust the light axis.
a
Binocular tube
(P. 18)
b
Diopter adjustment ring
(P. 18)
c
Condenser height adjustment knob
(P. 23)
d
Condenser centering knobs
(P. 23)
e
Field iris diaphragm lever
(P. 22)
Engage the objective to be used in the light
path and bring the specimen in focus.
7
Objective switching button
(P. 14)
8
Focusing knob (dial)
(P. 15)
Focus adjustment knob
Adjust the aperture iris and field iris diaphragms.
f
Aperture iris diaphragm lever
(P. 25)
e
Field iris diaphragm lever
(P. 22)
Engage the required filters.
3
Filter pocket
(P. 21)
Adjust the brightness.
9
Light intensity control buttons
(P. 13)
*
Usable when the DIA OBS setting is made in the IX2-BSW (Ver. 01.03 or later) software.