background image

Olympus FV1200 User guide 

June 23, 2015 

Olympus FV1200 MPE Microscope User Guide 

 

A.  Microscope Start Up

1.

 

Sign-in to the log book with the start time. 

2.

 

Turn on mercury lamp power supply 

 

by pushing the 

ON/OFF button and releasing it (The light should lights 
up in 

Blue

).  

3.

 

Turn on microscope controller 

and XY stage 

controller

.

 

 

4.

 

Turn on the touch panel controller

 and touch “Start 

Operation”. 

5.

 

Turn on both scanner controllers (MAIN and SIM) 

switch ON and then turn the key ON.  
 
 
 
 
 
 
 
 
 
 

 

 

6.

 

Turn on the computer if it is not on. 

7.

 

Enter user name/password to log on to Windows7.  

 
 
 
 
 

 

 

 

 

 

 

Содержание FV1200 MPE

Страница 1: ...supply by pushing the ON OFF button and releasing it The light should lights up in Blue 3 Turn on microscope controller and XY stage controller 4 Turn on the touch panel controller and touch Start Ope...

Страница 2: ...power supplies a Multi Ar laser Switch to ON and turn the Key to ON b MCPSU 405 440 473 559 635nm Switch ON c LD559 laser Switch on and WAIT UNTIL THE TEMPERATURE LIGHT STOPS BLINKING and THEN turn th...

Страница 3: ...to 1x With zoomed view you can select the scanning area by moving the blue lined box around Laser power control Checking the box will activate the laser The power can be adjusted by pointing the mouse...

Страница 4: ...e sample at 2us pixel because of its fast display refreshing rate this will be good for focusing and searching for ROIs without bleaching your sample Focus x4 will be faster but the display quality ma...

Страница 5: ...jective completely away from the sample and press again to bring the objective to the original position Fluorescent image observation 1 Select an objective lens using the touch panel controller TPC 2...

Страница 6: ...u selected If the Selected dyes upon opening the program are not ones that you want to use click the All Clear button first before selecting your dyes 2 Choose Image size default 512x512 from Size pan...

Страница 7: ...ckground in blue e Click to stop the fast scanning and click to scan at the imaging speed and to check the image quality Adjust more if needed 7 Repeat the above procedure for 515 nm EYFP and for 635...

Страница 8: ...one channel first and then scans the next channel If there is no bleed through between the emissions of some fluorophores you are using you can group them together to scan them simultaneously as a gro...

Страница 9: ...f the objective you are using so that there is no gap between the optical sections upon projection into 3D The step size can be fixed by checking the box 5 Click Stop button Adjust the Scan Speed if n...

Страница 10: ...icking My Image Folder button select Tools Option menu Click General tab and specify the path of your image folder with Browse button Upon creating a user account each user will have its own folder in...

Страница 11: ...pe for export ROI Overlay section allows saving the file with ROI information Output Format RGB Color the red green and blue channel will be saved in color as individual tif files in a folder RGB Colo...

Страница 12: ...hour or so leave the microscope system ON If not shut down the system as described below Shutting Off the system 1 Shut down the computer 2 Turn the mercury lamp off by pushing ON OFF button for two s...

Отзывы: