Olimpus FLUOVIEW FV500, Руководство пользователя, Страница: 464 / 470

TROUBLESHOOTING GUIDE
1-2
Page
Phenomenon Cause Treatment Manual Ref. Pages
1. Fluorescence image
cannot be observed The fluorescent dye and
excitation wavelength does
not match. Select a laser suitable for the
fluorescent dye method.
Change the ND filters by
reading 1-2-7, “Selecting the
ND Filters” in OPERATION
INSTRUCTIONS. 1-2-7, OPERATION
INSTRUCTIONS
The fluorescent dye does not
match the excitation dichroic
mirror, dichroic mirror and/or
barrier filter. Select the barrier filter
matching the fluorescent dye
method.
See 1-3-2-3, “Configuring
the Filters” in OPERATION
INSTRUCTIONS and follow
instructions of the [Optical
System Configuration]
window.
1-2-6 & 1-3-2-4,
OPERATION
INSTRUCTIONS
Focusing is not correct. Adjust focusing. 1-2-3, OPERATION
INSTRUCTIONS
The photomultiplier tube
voltage of the detector is low. Increase the photomultiplier
tube voltage using the [PMT]
scale in the [Acquire] panel. 2-2-1-4-9,
OPERATION
INSTRUCTIONS
The OFFSET value is too
high. Decrease OFFSET to an
optimum value. 2-2-1-4-9,
OPERATION
INSTRUCTIONS
The photomultiplier tube of
the channel set with the
detection mode setting slider
is not set. Check the [Ch1], [Ch2] ,
[Ch3] , [Ch4] or [Ch5] check
box in the [Acquire] panel. 1-2-8-3 & 2-2-1-4-3,
OPERATION
INSTRUCTIONS
2. Transmitted image
cannot be observed.
The transmittance detection
channel is not selected. In the [Acquire] panel, check
the check box of the [Ch]
with which “Transmitted” is
displayed. 2-2-3-3,
OPERATION
INSTRUCTIONS
The transmitted light filter is
engaged in the light path of
the microscope. Disengage the filter from the
light path. 1-3-2-4,
OPERATION
INSTRUCTIONS
3. Image is disturbed. The installation location is
subject to large vibrations. Consult Olympus. -
Darken the room before
acquiring image. - External light from a
fluorescent lamp, etc. is
detected.
Ensure that the top cover of
the scan unit is closed tightly. -
4. Image is striped. The laser reflection light is
penetrating. The barrier filter selection is
erroneous. 1-2-6 & 1-3-2-4,
OPERATION
INSTRUCTIONS
5. Image looks poor. The analyzer is engaged in
the light path. Disengage the analyzer from
the light path. 1-2-4, OPERATION
INSTRUCTIONS
The scan speed is high. Decrease the scan speed to
an optimum speed or
integrate the image. 1-2-8-9 & 2-2-1-6,
OPERATION
INSTRUCTIONS