Nikon N-STORM, Руководство по эксплуатации

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Chapter 3
The Screens of the N-STORM Software
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3.3.3 Channel Tab
A channel tab is used to change the display of multiple channels included in a dataset. Right-clicking a channel tab allows
you to set the property of the relevant channel. It is also possible to select whether to display the [Non Specific Activation]
channel and the [Z Rejected] channel.
No. Name Description
1 [All] Displays all the molecules for every channel. If the tabs of the
[Non Specific Activation] and [Z Rejected] channels are
displayed, the molecules in these channels are also included in
the [All] tab.
2 Fluorescent-probe-specific
channel tab
If an image is acquired on multiple channels, the
channel-specific tab is displayed.
3 [Non Specific Activation] In a cycle, molecules that are detected not in the first imaging
frame but in the second or subsequent imaging frame are
classified in a non-specific activation (NSA) channel.
Information on non-specific activation channels is used for
crosstalk subtraction.
4 [Z Rejected] As a result of 3D-STORM analysis, molecules that are plotted
outside the calibration range in the Z-axis direction, and
molecules whose ratio of X- and Y-axis direction image widths
exceeds the Max Axial Ratio of the Identification Settings are
classified in the Z Rejected channel. (For 3D-STORM only)
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