Nikon N-STORM, Руководство по эксплуатации

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Chapter 2
STORM Microscopy Operation
17
3 Finely and properly adjust the laser focal point of the
TIRF illuminator.
(1) Select the checkbox for the 647-nm laser.
(2) Turn on the Perfect Focus System (PFS) in [Ti Pad]
and set the focus.
(3) Finely adjust the laser focal point of the TIRF
illuminator so that the observation target is visible.
4 Check activation.
(1) Make sure that the checkbox for the reporter laser
(the figure on the right shows an example when a
647-nm laser is used) is selected, and then set the
power to 100% by adjusting the power slider.
(2) Make sure that the checkbox for the activation laser
(the figure on the right shows an example when a
405-nm laser is used) is deselected, and then set
the value to about 0.3% by adjusting the power
slider.
(3) Select the checkbox for the activation laser so that it
emits laser light for about a second, and then
immediately deselect it.
Check that the live image becomes bright once and
then returns to the previous state as a result of
activation causing the fluorescent probe to
fluoresce.
If activation cannot be checked
If emission of the activation laser does not make the live
image bright, this can be improved by replacing the
buffer solution of the specimen with a fresh solution.
3-(1)
3-(3)
3-(2)
4-(2)
4-(3)
4-(1)