Nikon N-STORM, Руководство по эксплуатации

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Chapter 2
STORM Microscopy Operation
31
7 Check the minimum intensity of bright points
(1) Click [Use Peak Statistics].
(2) Select the darkest bright point of all those to be
identified as molecules, and move the mouse
pointer onto its center. Read and write down the
value of [Peak Height]
.
(This value is used as the
minimum intensity for the identification of bright
points in the next step
.
)
8 Configure the settings for the identification of bright
points.
Click [Identification Settings].
In the dialog box, configure the settings as follows.
[Minimum Height]:
Specify the minimum intensity, checked in (2) of
the previous step.
[Maximum Height]: 20000
[CCD Baseline]: 100
Select the [3D] checkbox.
Click [>>] to configure the following detailed settings.
[Minimum Width (nm)]: 200
[Maximum Width (nm)]: 700
[Initial Fit Width (nm)]: 300
[Max Axial Ratio]:
2.5
[Max Displacement (pix)]: 0 (This allows bright points
recorded in multiple frames to be analyzed as
separate molecules, increasing the accuracy of
calibration.)
Click [OK].
Restore the Max Displacement setting
When performing analysis except for calibration, reset
[Max Displacement (pix)] to the default value of 1.
8
7-(2)
7-(1)