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Episcopic Microscopy

To perform episcopic microscopy, attach the LV-UEPI universal epi illuminator and a lamp for the
epi illumination to the microscope.

1. Switching the Episcopic Illumination

You can switch the illumination between the bright-
field and the dark-field to be used for the episcopic
microscopy by operating the illumination selector
lever on the right side of the epi illuminator.

When the lever is pushed in, the bright-field (BF)
illumination is specified. And when the lever is pulled
out, the dark-field (DF) illumination is specified. But
you cannot perform the episcopic dark-field
microscopy with this microscope. Note that the dark-
field setting (DF) is used for the diascopic
illumination.

ND8

NCB

F.S.

0.4

0.2

0.8

0.6

JAPAN

Achr

0.90

F

.

STOP

A

.

STOP

BF

DF

0

BINO

PH

OTO

PHO

TO

BINO &

Illumination selector lever

Lever position

Push in (BF)

Pull out (DF)

Illumination method

Episcopic bright-field illumination
(and Episcopic polarization microscopy)

Diascopic bright-field illumination
(The dark-field microscopy is not available.)

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Содержание Eclipse LV100POL

Страница 1: ...Polarizing Microscope ECLIPSE LV100POL Instructions M357 E 05 9 NF 1 ...

Страница 2: ......

Страница 3: ...efore using the product Do not discard the manual keep it handy for easy reference Safety instructions within this manual are accompanied by the following symbols to highlight their importance For your safety always follow the instructions accompanying these symbols Symbol Meaning Disregarding instructions accompanying this symbol may lead to serious injury or death Disregarding instructions accom...

Страница 4: ... VAC and 50 60 Hz Before connecting the power cord check that the power supply to be used conforms to the voltage and frequency described above Use of a non conforming power supply may result in equipment malfunction failure or fire 5 Power cord Be sure to use the specified power cord Using a wrong power cord may result in malfunctions or fire The product is classified as subject to Class I protec...

Страница 5: ...such as gasoline petroleum benzine paint thinner or alcohol near the lamp house while the lamp is lit or for about thirty minutes after it is turned off 8 Air vents Do not block the air vents on the microscope and lamp house If the air vents are blocked the temperature of the microscope will raise And it results in damage or fire 9 Reflection Lustrous samples reflect the illumination Do not observ...

Страница 6: ...ect sunlight or directly under a room light The image quality deteriorates if there is excessive ambient light Always install the microscope with a surrounding clear area of 10 cm or more Choose a location that is free from considerable dust or dirt Choose a flat surface with little vibration Choose a sturdy desk or table for the base of the microscopy system Do not install the microscope in a hot...

Страница 7: ... lamp house Never touch the glass surface of the lamp with bare hands Doing so will cause fingerprints grease etc to burn onto the lamp surface reducing the illumination If you do get any fingerprints or dirt on the lamp wipe them clean Make sure the lamp house cover is securely fitted to the lamp house after replacing lamps Never turn on the lamp with the lamp house cover removed When you dispose...

Страница 8: ...on 24 4 Coarse Focus Knob Fine Focus Knob Operation 25 5 Stage Rotation 26 6 Trinocular Eyepiece Tube Operation 27 7 Diopter Adjustment 28 8 Interpupillary Distance Adjustment 28 9 Condenser Operation 29 10 Focusing and Centering the Condenser 30 11 Adjusting the Aperture Diaphragm 30 12 Adjusting the Field Diaphragm 32 13 Filter on the Field Lens 32 14 Centering the Objective 33 15 Oil Immersion ...

Страница 9: ...e Polarizing Intermediate Tube 53 8 Attaching the Eyepiece Tube 53 9 Attaching Eyepieces 53 10 Attaching a Camera for the Trinocular Eyepiece Tube 54 11 Attaching Eye Level Risers 54 12 Connecting the Power Cord 55 Troubleshooting 56 1 Viewing Problems and Control Problems 56 2 Electrical System Problems 59 Care and Maintenance 60 1 Cleaning Lenses and Filters 61 2 Cleaning the Painted Plastic and...

Страница 10: ... Specimen holder 2 P CL 1 4 λ tint plate 1 Stage rotation clamp screw Power indicator Power cable Brightness control knob Epi dia selection switch Selection switch between episcopic illumination and diascopic illumination Epi dia indicator Coarse torque adjustment ring TORQUE 45 click stop lever Condensor focus knob Binocular section Polarizer for the diascopic microscopy placed in the polarizer r...

Страница 11: ...enser top lens Slot 20 x 6 mm Used for parts available on the market such as a compensator or so on Objective centering screws Field lens Lamp cable Field diaphragm control Filter selector switches Fine focus knob Coarse focus knob Coarse focus stopper ring CLAMP Optical path selector lever Analyzer slider Bertrand lens centering screws both sides Photomicrographic equipment clamp screw Diopter ad...

Страница 12: ...er This part is used to perform the episcopic polarization microscopy F S ND8 NCB 0 4 0 2 0 8 0 6 JAPAN Achr 0 90 F STOP A STOP BF DF 0 BIN O PHOTO PHO TO BIN O Epi illuminator LV UEPI Polarizer slider Field diaphragm centering screw Aperture diaphragm open close lever CAUTION for heat symbol Episcopic illumination lamp house Diascopic illumination lamp house Lamp cable Field diaphragm open close ...

Страница 13: ...ing the lamp Wait for at least 30 minutes after turning off the lamp Use 12V50W HALOGEN lamp only 1 2 3 LV LH50PC JAPAN 6 5 2 7 0 2 LAMP DC12V 50W ECLIPSE LV100POL 100 240V 1 2A 50 60Hz MADE IN JAPAN including interference that may cause undesired operation 8 5 0 0 0 1 This Class A digital apparatus complies with Canadian ICES 003 Cet appareil numérique de la classe A est confirme à la norme NMB 0...

Страница 14: ... 42 3 Remove the polarizer for the diascopic microscopy See Page 36 4 Push the epi dia selection switch to select the diascopic illumination The indicator for the diascopic illumination lower side turns on See Page 23 5 Turn the brightness control knob to adjust the brightness When the brightness control knob is turned the brightness of the illumination selected by the epi dia selection switch is ...

Страница 15: ...he condenser as high as it will go 12 Fully open the field diaphragm and the condenser aperture diaphragm ND8 NCB F S 0 4 0 2 0 8 0 6 JAPAN Achr 0 90 0 6 Set the appropriate filter into the optical path 0 BINO PHO TO PHOT O BINO 7 Remove the analyzer from the optical path 8 Remove the Bertrand lens from the optical path 0 4 0 2 0 8 0 6 JAPAN Achr 0 90 0 9 Set the 10x objective 10 Set the specimen ...

Страница 16: ...ge 25 16 Center the objective See Page 33 17 Focus and center the condenser See Page 30 0 BINO PHO TO PHOT O BINO 13 Set to the BINO position ND8 NCB F S 0 4 0 2 0 8 0 6 JAPAN Achr 0 90 0 15 Loosen the coarse focus stopper ring and focus on the specimen 0 BINO PHO TO PHOT O BINO 14 Adjust the interpupillary distance 14 Adjust the diopters 0 4 0 2 0 8 0 6 JAPAN Achr 0 90 0 16 Centering the objectiv...

Страница 17: ...es 31 and 32 Focus on the specimen again using the fine focus knob or the coarse focus knob See Page 25 The top lens of the swing out condenser must be operated depending on the magnification of the objective to be used See Page 29 To use an oil immersion type objective or a water immersion type objective perform the oil immersion or water immersion See Pages 34 and 35 19 When the microscopy ends ...

Страница 18: ...the polarizing intermediate tube to locate the analyzer into the optical path Set the scale for the analyzer to the 0 position And remove the Bertrand lens from the optical path See Pages 37 and 39 3 Set the polarizer for the diascopic microscopy into the polarizer rotation ring See Page 36 4 Adjust the orientation of the polarizer and the analyzer See Page 38 After the adjustment observe the spec...

Страница 19: ...re diaphragm should be adjusted so that its image circumscribes the conoscopic view field or should be fully opened The field diaphragm should be adjusted so that its image circumscribes the conoscopic view field The top lens of the P swing out condenser must be placed in the optical path See Page 29 The table below shows the settings for the orthoscopic microscopy and the conoscopic microscopy 0 ...

Страница 20: ...indicator on the microscope base is lit See Page 22 2 Push the epi dia selection switch to select the episcopic illumination The indicator for the episcopic illumination upper side turns on See Page 23 3 Turn the brightness control knob to adjust the brightness When the brightness control knob is turned the brightness of the illumination selected by the epi dia selection switch is changed See Page...

Страница 21: ... goes through the binocular eyepiece See Page 27 13 Adjust the diopters and the interpupillary distance See Page 28 14 Focus on the specimen Fully loosen the coarse focus stopper ring See Page 25 15 Center the objective See Page 33 16 Center the field diaphragm See Page 43 ND8 NCB F S 0 4 0 2 0 8 0 6 JAPAN Achr 0 90 F STOP A STOP BF DF 0 BINO PHO TO PHOT O BINO 6 Remove the analyzer from the optic...

Страница 22: ...on See Pages 34 and 35 18 To perform the episcopic polarization microscopy locate the polarizer on the side of the epi illuminator into the optical path and locate the analyzer on the polarizing intermediate tube into the optical path The polarizer azimuth should be adjusted with the ring on the polarizer slider See Page 36 19 To return to the bright field microscopy Fully rotate the brightness co...

Страница 23: ...ailed information refer to the instruction manual provided with the camera When using the DS L1 you must choose and enter at least the following information Folder for data storage Name of the file to be saved You can select Auto File format and file size Date and destination of data 4 Select the camera scene mode suitable for the microscopy method 5 Set the camera white balance To adjust the whit...

Страница 24: ...gen lamp With the specified lamp house LV LH50PC the lamp lights up simultaneously as the power supply for the microscope is turned on Power supply for the lamp To use a lamp other than the specified one turn on the power supply for the light source by hand High Temperature CAUTION HALOGEN 12V50W Do not touch the lamphouse while the lamp is lit The surface of the lamphouse becomes hot when the lam...

Страница 25: ...p Turn the brightness control knob to the far side counter clockwise and position to the OFF to turn off the illumination selected by the epi dia selector switch When an external light source is used the illumination is turned on off at the light source side Color temperature shift affected by the brightness control Adjusting brightness with the brightness control knob will affect the lamp color t...

Страница 26: ...nce filter IF interference filter Intended use For color balance adjustment and color photomicrography For brightness adjustment transmittance 25 For brightness adjustment transmittance 6 For contrast adjustment For interference For the episcopic illumination Two filter sliders are located near the rear side of the epi illuminator Each slider can holds two filters Push in or pull out the filter sl...

Страница 27: ...he stage cannot be raised higher than the position the operator specifies When the coarse focus stopper ring is rotated in the direction of the arrow labeled CLAMP on the microscope base the coarse focus knob cannot be used to move the stage any higher Movement of the stage by the fine focus knob is not restricted For example once the coarse focus knob is clamped in place at the focus position a r...

Страница 28: ...ick stop function where the stage has dropped into a click position If the click stop function is released where the stage is not at this position the first 45 increment will not be accurate the next time the click stop function is operated Attachable Mechanical Stage Option The optional attachable mechanical stage is installed by inserting the two pins on the bottom into the two pinholes on the s...

Страница 29: ...r the binocular part is 20 and the distribution of light for the vertical tube is 80 Vertical tube adapter The TV vertical tube adapter or the TV vertical tube adapter 0 55x can be mounted on the vertical tube section of the trinocular eyepiece tube To mount the adapter remove the cap from the vertical tube section of the trinocular eyepiece tube insert the vertical tube adapter and fix it with th...

Страница 30: ...the left eye Turn the diopter adjustment ring on the eyepiece to bring the specimen into focus not the fine coarse focus adjustment knob 8 Interpupillary Distance Adjustment Interpupillary distance adjustment improves the ease of binocular observation Perform steps 1 to 14 on Page 12 Diascopic Bright Field Microscopy and focus on the specimen using the 10x objective Then move the eyepiece sleeve u...

Страница 31: ...py the top lens is placed into the optical path During measurement of retardation or evaluation by interference color swing out the top lens the condenser aperture diaphragm may be stopped down and illuminate with light that is as parallel to the optical axis as possible Depending on the type of objective the indicated numerical aperture of the objective may not be achieved For example when an obj...

Страница 32: ...cal path Turn the fine focus knob to focus on the specimen 6 Turn the condenser focus knob to form the field diaphragm image on the specimen surface 7 Adjust the field diaphragm control and the condenser centering screws so that the field diaphragm image inscribes the view field 8 To observe the specimen turn the field diaphragm control so that the field diaphragm image circumscribes the view fiel...

Страница 33: ...m is stopped down too far the resolution is reduced therefore except when viewing a nearly transparent specimen we do not recommend stopping down the aperture to less than 60 of the numerical aperture of the objective Adjusting the size of the aperture diaphragm according to the condenser scale Since the condenser scale indicates the numerical aperture adjust the aperture diaphragm ring according ...

Страница 34: ...ge capturing In general good results will be obtained by stopping down the field diaphragm to settings slightly wider than the area to be reproduced within the photo frame or monitor display For the field diaphragm of the epi illuminator refer to Page 42 22 Episcopic Microscopy 13 Filter on the Field Lens You can attach up to two optional filters of 45 mm diameter into the filter pocket of the fie...

Страница 35: ...ropriate target such as granules that can be easily used as a marker in the specimen to the center of the crosshairs of the eyepiece 3 Insert the centering tools into the centering screws on the nosepiece 4 Rotate the stage about 180 degree Move the objective using the centering tools so that the center of the crosshairs moves by one half the amount of movement of the target 5 Move the specimen an...

Страница 36: ...e oil and replace it with new oil Use as little oil as possible just enough to fill the space between the tip of the objective and the specimen or between the tip of the condenser and the specimen If too much oil is applied the excess oil will flow onto the stage or around the condenser Wipe off oil Any oil remaining on the oil immersion objective or adhering to the dry type objective will noticea...

Страница 37: ...the same as for oil immersion type objectives Since water evaporates readily monitor the immersion water during observation Avoid using too much water since excess water will flow onto the stage and around the condenser promoting corrosion Wipe off water After use wipe off water from the tip of the objective and condenser then follow up by wiping with absolute alcohol If you observe water stains a...

Страница 38: ...olarizer for the episcopic microscopy into the epi illuminator Insert the polarizer slider with the orientation index facing front side eyepiece side Pushing the polarizer slider in to the first click stop position inserts the empty hole into the optical path Pushing it further in to the second click stop position inserts the polarizer into the optical path You can change the polarizer orientation...

Страница 39: ...er case its scale displays the opposite way Be careful Rotate the analyzer The analyzer slider has a rotating dial The orientation of the analyzer can be rotated with it To adjust the analyzer loosen the analyzer rotation clamp screw and rotate the rotating dial The angle of rotation can be read from 0 to 180 degrees in steps of 0 1 degrees with the two vernier scales The intermediate tube also ha...

Страница 40: ...tion as that of the polarizer in some commercially available technical manuals and reference books For the episcopic observation 1 Push in the analyzer setting knob and move the analyzer out of the optical path 2 Place a dummy specimen on the stage It must have high reflectance with optical isotropy for example a mirror And then focus on the specimen 3 Pull out the analyzer slider and move the ana...

Страница 41: ...e case that you have centered the objective already Page 33 In this adjustment the aperture diaphragm image is used in the same manner as the condenser lens adjustment Do as follows 1 Perform steps in Page 12 1 Diascopic Bright Field Microscopy and focus on the specimen And focus and center the condenser Page 30 2 Move the Bertrand lens into the optical path 3 Stop down the aperture diaphragm of t...

Страница 42: ...specimen P CS Senarmont compensator Remove the P CL 1 4 λ tint plate from the slot of the polarizing intermediated tube and insert the P CS Senarmont compensator into the slot You can measure retardation of light up to 1 λ with the following steps 1 Determination of extinction position Rotate the stage with the specimen under the crossed Nicols to find the direction where the part of the specimen ...

Страница 43: ... the P CQ quartz wedge into the slot of the intermediate tube and confirm that the interference color of the section of the specimen to be measured changes toward the lower order side If the interference color changes toward the higher order side rotate the stage another 90 degree 3 Measurement Move the section of the specimen to be measured to the center of the crosshairs of the eyepiece Next sli...

Страница 44: ... pushed in the bright field BF illumination is specified And when the lever is pulled out the dark field DF illumination is specified But you cannot perform the episcopic dark field microscopy with this microscope Note that the dark field setting DF is used for the diascopic illumination ND8 NCB F S 0 4 0 2 0 8 0 6 JAPAN Achr 0 90 F STOP A STOP BF DF 0 BINO PHO TO PHOT O BINO Illumination selector...

Страница 45: ...uced within the photo frame or monitor display Centering the field diaphragm 1 Perform steps in Page 18 4 Episcopic Microscopy with the Epi Illuminator Option and focus on the specimen using the 10x objective under the episcopic illumination 2 Lower the field diaphragm open close lever to stop down the field diaphragm 3 Turn the field diaphragm centering screws on both sides so that the field diap...

Страница 46: ...ng the exit pupil of the objective the bright area when the aperture diaphragm is fully opened in the eyepiece tube The diaphragm image may not appear in the case of samples with low reflectivity In this case change to a sample with a near polished surface The aperture diaphragm in the LV UEPI illuminator was centered before shipping You don t need center the aperture diaphragm 4 Operating Filters...

Страница 47: ... as necessary Adjusting the condenser Focus and center the condenser always For normal operations set the diaphragm aperture to 70 to 80 of the N A of the objective Confirming the photomicrographic range The image on the monitor represents the photomicrographic range Confirming the focus Check the focus by viewing through the eyepiece and viewing the monitor If the focal positions for the two imag...

Страница 48: ...r objectives may loose accuracy when exposed to even a weak physical shock Required tools Two hexagonal screwdrivers 2 mm provided with the microscope One hexagonal wrench 2 5 mm and one hexagonal wrench 3 mm provided with the microscope When not using place these in the tool holder at the right side of the microscope base Installation location This microscope system is a precision optical instrum...

Страница 49: ...4 λ tint plate P CS Senarmont compensator P CQ quartz wedge Trinocular eyepiece tube Eyepiece TV vertical adapter TV vertical adapter 0 55x or Circular graduated stage Epi illuminator P swing out condenser Polarizer or Power cord Lamp cable Polarizing intermediate tube with analyzer slider 12 V 50 W halogen lamp Lamp house Various filters Polarizer slider Filter slider x2 Attachable mechanical sta...

Страница 50: ...n about the optional attachable mechanical stage see Page 26 Attachable Mechanical Stage Option 2 Attaching the Condenser To perform the polarization microscopy the P swing out condenser must be attached 1 Turn the coarse focus knob until the elevating section is raised to the uppermost position 2 Turn the condenser focus knob until the condenser holder is brought to the lowermost position 3 Inser...

Страница 51: ...age fully and remove the specimen and all objectives Then hold the nosepiece by hand to prevent falling and remove the nosepiece 4 Attaching Objectives To perform the polarization microscopy objectives exclusively designed for the polarization microscopy must be attached Lower the stage completely Screw objectives into the nosepiece so that the magnification increases with the clockwise rotation a...

Страница 52: ...my sliders slide them in to the limit so that the empty hole will be set in the optical path Filter sliders and filters 1 Remove each filter slider from the illuminator Two filter sliders are used for the epi illuminator 2 Pull out the locking plate from the filter slider 3 Insert the desired filter Two filters can be set on the filter sliders 4 Reinstall the locking plate to its original position...

Страница 53: ...mp house after replacing lamps Never turn on the lamp with the lamp house cover removed When you dispose of the replaced lamp do not break it up Instead dispose of the used lamp as special industrial waste or dispose of it according to the local regulations and rules 1 Attaching the lamp house Before performing the following procedures turn off the power supply for the microscope press the side an...

Страница 54: ...over clamp screw using the hexagonal wrench 2 Remove the lamp house cover 3 Push down the lamp clamp lever and remove the old lamp 4 With the lamp clamp lever held down insert the electrodes of a new lamp into the holes of the socket Insert the lamp as far as it goes and then release the lamp clamp lever to secure the lamp Be careful not to touch the glass surface of the lamp with bare hands When ...

Страница 55: ...in the polarizing intermediate tube while rotating in the clockwise direction 8 Attaching the Eyepiece Tube Loosen the eyepiece tube clamp screw on the intermediate tube with the provided tool sufficiently Attach the eyepiece tube onto the mount part on the illuminator and fix it with eyepiece tube clamp screw using the hexagonal screwdriver Caution to remove the eyepiece tube Hold the eyepiece tu...

Страница 56: ...he suitable adapter for the camera onto the tip of the TV vertical tube adapter 3 Attach the camera head to the adapter tip 4 Attach the camera cable to the camera head Adjust the camera head position before using the camera 11 Attaching Eye Level Risers Optional eye level risers are used for the adjustment of the height of the eyepiece tube to fit the observer s eye point Up to two eye level rise...

Страница 57: ...o Class I protection against electrical shock Make sure it is connected to an appropriate ground terminal protective earth terminal For specifications of the power cord refer to VII Specification Turn off the power switch on the microscope flip it to the side Insert the socket of the power cord into the AC inlet on the back of the microscope Then securely plug the power cord to the wall outlet ...

Страница 58: ...e condenser focus knob so that the field diaphragm image is focused on the specimen surface p 30 Center the condenser p 30 Install the condenser correctly p 48 Move the top lens to the correct position p 29 Cause The lamp is not attached correctly The optical path selector lever on the eyepiece tube is in an intermediate position The optical path selector lever on the eyepiece tube is not set to 1...

Страница 59: ...e on a flat and level surface Center the condenser P 30 Locate the NCB 11 filter into the optical path P 24 Rotate the brightness control knob to increase the intensity of the light source and adjust the brightness with ND filters P 23 and 24 Adjust the brightness with the brightness control knob Or locate a ND filter into the optical path P 23 and 24 Cause The aperture diaphragm is stopped down t...

Страница 60: ...ied type of cover glass thickness 0 17 mm Secure the specimen holder correctly Adjust the interpulillary distance P 28 Adjust the diopters P 28 Adjust the interpulillary distance P 28 Adjust the diopters P 28 Adjust the brightness with the brightness control knob or the combination of ND filters P 23 and 24 Use eyepieces having the same viewfield number Cause The lamp voltage is too low A ND filte...

Страница 61: ... is attached The lamp is blown A wrong lamp is used The lamp is about to blow The power cable or the cable of the lamp house is not connected securely The lamp is not securely inserted into the socket The lamp house is not connected securely 2 Electrical System Problems Problem The coarse focus knob is heavy in rotation The stage falls on its own weight and the image goes out of focus The stage ca...

Страница 62: ... as eyepieces and glass plate according to the procedure described in 1 Cleaning Lenses and Filters without removing them from the microscope Nikon recommends cleaning them frequently Clean the objectives filters and the like to maintain the optical performance When cleaning the objectives remove them from the microscope Clean them whenever they are contaminated Microscopes and stages are contamin...

Страница 63: ...olute alcohol 2 Cleaning the Painted Plastic and Printed Parts Do not use organic solvents such as alcohol ether or paint thinner on painted components plastic components or printed components Doing so could result in discoloration or in peeling of the printed characters For persistent dirt dampen a piece of gauze with neutral detergent and wipe gently 3 Storage Store this product in a dry place w...

Страница 64: ...echanism Coarse focus knob 14 mm revolution Fine focus knob 0 1 mm revolution 10x field number 22 Circular graduated stage Provided with two verniers Provided with a rotation mechanism with a clamp Provided with 45 click stop mechanism Provided with two specimen holders Quintuple type five sockets Provided with objective centering mechanism Input voltage 100 240 VAC 10 50 60 Hz Rated current 1 2 A...

Страница 65: ...gy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense This class A digital apparatus complies with Canadian ICES 003 Cet appreil numérique de class...

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