29
New Brunswick Scientific
User’s Guide
4
4
R
R
O
O
U
U
T
T
I
I
N
N
E
E
M
M
A
A
I
I
N
N
T
T
E
E
N
N
A
A
N
N
C
C
E
E
4.1 General
Notes
To ensure that chamber conditions remain as stable as possible, be sure to minimize
the length of time that the inner door is open. The magnetic door catches are
specifically designed to make door opening and closing as easy as possible. When
you open the inner door, wipe off any small drops of condensate that may have
formed on the inner seal. This will avoid a build-up of condensation.
If you are using the humidity tray for humidification, be sure to follow the indications
provided
in Section 3.3.
4.2 Daily
Checks
1. Check that the temperature and CO
2
levels are reading within specification.
2. Check the reserve pressure in the CO
2
cylinder (normally 725psi or 50 bar when
full). The design of the incubator ensures very low consumption of CO
2
: during
normal working conditions, a typical large cylinder should last approximately 12
months. If there is a significant drop at the cylinder pressure of 725psi or 50 bar,
it means that the cylinder is almost empty and should be replaced. Ensuring that
there are no leaks at any of the connections will ensure a greater lifetime to the
CO
2
supply and will help avoid accidentally running out of CO
2
.
4.3 Monthly
Checks
1. Refill the humidity tray (to a maximum volume of 1.5 liters). The use of warm
water (
∼
37.0°C) will ensure a rapid return to optimum chamber conditions.
After refilling, check that the humidity tray is situated directly beneath the
deflector plate on the rear wall of the chamber. This ensures that any condensate
collected is returned to the reservoir.
2. If required, you can take a sample of the gas inside the chamber using the CO
2
sample port, and check it using a CO
2
gas analyzer. The CO
2
sample port is
located on the left-hand side of the rear panel (
see Figure 3
).
If you conduct a sampling, please ensure the following:
Turn off the CO
2
gas by re-programming the setpoint for CO
2
to 0.2% to
prevent CO
2
from being injected into the chamber and giving a false reading.
A flow rate
≤
0.5 liters/minute is used to take a sample.
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