21
New Brunswick Scientific
User’s Guide
3.3 Using the Humidity Tray
1. Fill the humidity tray with 1.5 liters of warm (
∼
37.0ºC) distilled water.
2.
For cell culture work
, we recommend the addition of one small teaspoonful
(~0.11oz or 3.2g) of copper sulphate (or another recognized biocide) in the
humidity tray. Tests have shown that in addition to inhibiting bacterial growth in
the tray, this can reduce contamination on the chamber walls.
3.
For IVF and other sensitive work
, we do not recommend the use of any biocide in
the humidity tray. To reduce the possibility of contamination, every 10 - 14 days
the humidity tray water should be changed and the humidity tray should be
cleaned with a solution of 70% isopropyl alcohol and 30% distilled water.
NOTE:
Ensure that the Humidity Tray is pushed fully back against the rear wall.
The humidity tray is designed so that it is always in the optimum
position for condensate collection.
4. Let the incubator run for at least two hours (preferably overnight) to allow
conditions to stabilize.
3.4 Setting Temperature & CO
2
At this time, program the desired Temperature and CO
2
setpoints. Temperature and
CO
2
may be set within the following ranges:
Table 4: Temperature & CO
2
Setpoint Ranges
Parameter
Available Setpoint Range
Temperature
10ºC to 50ºC
(must be at least 1ºC above ambient)
CO
2
0.2% to 20%
To set the Temperature:
1. Press the Programming (
∗
) key.
CAUTION!
Never leave water in the humidity tray if the incubator is
switched OFF, as this may cause damage to the CO
2
sensor.
Содержание CO-150
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