NanoEnTek EVE, Руководство пользователя

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EVE
TM
, Automatic Cell Counter ©2013 NanoEnTek Inc. 35
Troubleshooting
Inaccurate cell count
Sample handling
Low and high readings
Poor image quality
Cell clumping
Error codes
∙
Do not insert the EVE™ Cell counting slide upside-down
as this may introduce liquid into the instrument that could
damage it.
∙
Do not reuse the EVE™ Cell counting slides, as leftover
dye from the previous reading may affect the next reading.
∙
Do not use any other counting slides such as a glass
hemocytometer with the EVE™ as it results in inaccurate
cell count and may damage the instrument.
∙
Ensure that the sample covers the entire counting area
and the EVE™ Cell counting slide is inserted completely
into the counter.
∙
The EVE™ is designed to read samples from 1 × 10
4
cells/
mL to 1 × 10
7
cells/mL, with the highest accuracy between 1
× 10
5
cells/mL and 4 × 10
6
cells/mL.
If your sample is not in this range, you may need to dilute
the sample or add more cells and read the sample again.
∙
While viewing cells in the Zoom mode, use the focus knob
to adjust the image to ensure that live cells have bright
centers, and dead cells have dark/blue centers.
∙
Ensure the cells are not clumped.
∙
To maintain instrument sensitivity, we recommend that you
calibrate the counter each year as described on page 24.
∙
See page 37 for a description of error codes.