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NanoEnTek EVE, Руководство пользователя

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NanoEnTek EVE Скачать руководство пользователя страница 24

24

Operation

Calibrating

1. Press 

Settings

 and then press 

Calibration button

.

2.  Check the current background level and press 

Yes button

 to 

recalibrate.

3.  To recalibrate the EVE™, mix 10 µL trypan blue solution with 
10 µL of a standard buffer, such as phosphate buffered saline 
(PBS). Mix thoroughly. 

Содержание EVE

Страница 1: ...User Manual EVE Automatic cell counter...

Страница 2: ...factured by NanoEnTek Inc HQ 12F 5 Digital ro 26 gil Guro gu Seoul 152 740 Korea Tel 82 2 6220 7940 Fax 82 2 6220 7721 The information in this manual is described as accurately as possible Firmware an...

Страница 3: ...ferring data to a computer Using the beads mode for counting beads Calibrating PC software Installation Open the data Cell gating Counting Spread sheet Prepare report Cleaning and maintenance Troubles...

Страница 4: ...are missing or damaged contact your local distributor or e mail sales nanoentek com Automatic cell counter 1 EA USB drive 4 GB 1 EA User manual 1 EA Power cord with 4 adaptor cords 4 pcs set for U S C...

Страница 5: ...is compatible with a wide variety of eukaryotic cells and provides information on cell size The EVE offers an intuitive user interface and provides the option to save and print cell count data using t...

Страница 6: ...res cell concentrations ranging from 1 104 to 1 107 cells mL and cells with sizes ranging from 5 m to 60 m Provide the clumping cell counting function to get more accurate results using EVETM PC softw...

Страница 7: ...displays data from the cell count The slide port is used to insert the EVE Cell counting slide containing the sample with trypan blue stain into the counter for analysis The USB port allows you to tr...

Страница 8: ...ast between live bright centers and dead dark blue centers cells This is important to obtain accurate cell counts and viability measurements The focus lock knob is used to lock the image adjustment fo...

Страница 9: ...mage the instrument 5 When the instrument is turned on the Start Up screen is displayed Here you can proceed immediately to cell counting set up the instrument for cell or bead count or adjust the scr...

Страница 10: ...perate the instrument for cell counting choose Cells or bead counting choose Beads Parameters see below and next page for details Calibration to calibrate the instrument page 24 Update to install new...

Страница 11: ...count Adjusting the number up increases inclusiveness thereby decreasing the lower cell size range e g 50 of 15 is 7 5 m 15 7 5 7 5 m Maximum cell size is used to determine the high range of cell size...

Страница 12: ...nce a protocol is saved it is available for use at any time Press Load protocol button 6 The protocol appears in the protocol menu Use the up and down arrows to find your saved protocol To use the pro...

Страница 13: ...month using the arrow keys and then press the day on the calendar Use a pointed object like a stylus or pipette tip to push the small buttons on the calendar 4 To select the time scroll to the approp...

Страница 14: ...lls within 3 minutes of mixing the cells with trypan blue solution as trypan blue is toxic to cells For best data with biological samples we recommend counting at least two samples and taking an avera...

Страница 15: ...ent The Start up screen is displayed 2 The EVE is preset to Cells mode 3 Mix well the 10 L of your sample and the 0 4 trypan blue stain using a pipette 4 Load 10 L of the sample mixture on EVE Cell co...

Страница 16: ...h chamber is counted separately Auto focusing AF on mode is set as a default If necessary to personalize focus turn on the manual focusing AF off mode in the Setting menu Refer to page 10 In case of A...

Страница 17: ...ike to see on the grid 7 2 While viewing cells in the zoom mode use the Focus knob to adjust the image For information on how to check counting results refer to the page 19 After you have counted the...

Страница 18: ...ight centers and dark edges Dead cells have a uniform blue color throughout the cell with no bright centers Correct Image Incorrect Image 1 Dead cells have bright blue centers are counted as the live...

Страница 19: ...each sample and the cell count for live dead and total cells as well as percentage viability is displayed on the screen Record the cell count or insert a USB drive and press Save button see page 21 1...

Страница 20: ...nd repeat the procedure 14 The EVE USB drive holds one set of data To save your data for future analysis or archiving you must record the data or save after each reading See page 21 for Transferring d...

Страница 21: ...ing data are saved 3 Enter the file name using the keypad buttons displayed on the Save menu The numerical data is also automatically saved as a CSV file that can be opened with any spreadsheet progra...

Страница 22: ...and down 3 Load 10 L of the sample mixture on EVETM Cell counting slide side A or B using pipette The two chambers of the slide are labeled A and B for easy tracking of your samples 4 Insert the EVE C...

Страница 23: ...ads button 8 The instrument takes approximately 20 seconds to count each sample and the bead count is displayed in the screen Record the bead count 9 To count beads in the other side of the slide cham...

Страница 24: ...d then press Calibration button 2 Check the current background level and press Yes button to recalibrate 3 To recalibrate the EVE mix 10 L trypan blue solution with 10 L of a standard buffer such as p...

Страница 25: ...10 L of the sample mixture to the chamber ports on one side of the EVE Cell counting slide Press Start 5 After calibration is completed press the OK button to restart the instrument and proceed to cel...

Страница 26: ...erred to your computer by the USB drive and imported into any spreadsheet program without the need for EVE PC software Computer requirements EVE is not compatible with Macintosh operating systems USB...

Страница 27: ...Installation 4 The computer activates the installation of the Software 5 Click OK when Installation is complete 6 When the software is installed the PC software icon appears on your desktop 7 Click on...

Страница 28: ...n different condition 3 Adjust the cell image by pressing the Zoom button Navigate the fields by clicking the location you like to see on the grid 4 If it is necessary you can adjust cell counting res...

Страница 29: ...o make the changes 6 You can check cell size graph Cell size graph will be shown with different color for viable cells dead cells and total cells Using each button you can check individual result for...

Страница 30: ...ize setting the algorithm calculates the smallest object size to include in the final measurement Adjusting the number up increases inclusiveness thereby decreasing the lower cell size range Maximum c...

Страница 31: ...lect the saved file you wish to open and click Counting button for re counting the cells exception algorithm will solve this problem User can achieve more accurate result 2 You can check more accurate...

Страница 32: ...Spread sheet 1 Select the saved file you wish to open and click Spread sheet button 2 User can check cell distribution according to cell size differences And also this information can be exported by...

Страница 33: ...you wish to open and click Prepare report button 2 You can check preview of report before export to PDF file 3 Click Export to PDF button to obtain a printable version of the image and data 4 Type th...

Страница 34: ...a soft cloth lightly moistened with LCD cleansing detergent Cleaning the screen with excessive force can damage the LCD the screen Wipe the screen dry immediately Do not reuse the cell counting slide...

Страница 35: ...instrument Ensure that the sample covers the entire counting area and the EVE Cell counting slide is inserted completely into the counter The EVE is designed to read samples from 1 104 cells mL to 1...

Страница 36: ...ve a USB drive when saving or reading data as it may damage the counter Download a new version of the firmware from the website on a different USB drive and try updating the firmware on the EVE Contac...

Страница 37: ...ffer memory allocation fail Camera memory allocation fail Message System Error E0101 Reboot the device or refer to service personnel Error code 0101 0102 0103 0104 0201 0301 0302 This section describe...

Страница 38: ...caused by substituting alternative parts 4 Use of fittings or spare parts supplied by anyone other than NanoEnTek 5 Damage caused by accident or misuse 6 Damage caused by disaster 7 Corrosion caused...

Страница 39: ...rone to vibrations which induces the risk of malfunction or damage of the instrument Never insert any objects into the air vents of the instrument as this can result in electric shock personal injury...

Страница 40: ...ins rez jamais aucun objet dans les orifices d a ration de l instrument car cela pourrait entra ner un choc lectrique des blessures chez les utilisateurs et des dommages d quipement Branchez le cordo...

Страница 41: ...ccordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the...

Страница 42: ...easurement range cells mL Cell size range Dimensions Weight Material Dimensions Chamber depth Chamber volume EVE USB drive AC 100 240 VAC 1 5A 50 60 Hz 12 VDC 1 0 A Indoor use only 5 40 C 20 80 2 000...

Страница 43: ...o EVS 050 EVS 1000 EVS 5000 EBB 001 Contents 50 slides 100 counts with 1 ea X 1 5 mL of trypan blue 0 4 1 000 slides 2 000 counts with 20 ea X 1 5 mL of trypan blue 0 4 5 000 slides 10 000 counts with...

Страница 44: ...ional product information and special offers For more information or technical assistance please call or email NanoEnTek Inc HQ 12F 5 Digital ro 26 gil Guro gu Seoul 152 740 Korea Tel 82 2 6220 7940 F...

Страница 45: ...EVE Automatic cell counter NESMU EVE 001E V 1 5...

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