Nac HotShot, Руководство пользователя

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Average Intensity Over Time
This function is very similar to the above function, but instead of just finding out a
specific pixel’s value, this function will work out the average intensity for a row,
column or area on each image within the ranges set. To use this function on a row or
column:
1. Select the channel via the ‘Display Options for All Tools’ box.
2. Select whether a row, column or area is required.
3. Select the range required by moving the mark IN and mark OUT slider
triangles or inputting the frame number direct.
4. Clicking the Pick Pixel’ button.
5. The click the pixel on the image which the row or column goes through.
6. The progress bar will indicate the progress of the function.
7. To cancel this function at any time, click the ‘Cancel’ button.
8. When the programme has finished gathering the results, a new window
displaying the average intensities over time for the row column.
To use an area of interest follow the above except select the ‘Area of Interest’ bullet
and then drag the area of interest on the image after clicking the ‘Pick Pixel’.