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SpectraMax MiniMax Imaging Cytometer Installation Guide

20

5024111 C

Item

Description

1 

Power switch

2 

Off

3 

On

Figure 1-13: Power Switch

To connect the cytometer cables:

1. Make sure that the instrument and host computer are placed on a dry, flat work

area with sufficient space for the two devices and the required cables. To ensure
sufficient ventilation and provide access for disconnecting power from the
instrument, maintain a 20 cm to 30 cm (7.9 in. to 11.8 in.) gap between the rear of
the instrument and the wall.

2. Make sure that the power switch on the rear of the microplate reader is in the

Off position.

3. Turn on the power to the host computer.
4. Install the SoftMax Pro Software on the computer. See the SoftMax Pro Software

installation guide or user guide.
The instrument is supported by SoftMax Pro Software version 6.3 or later. The

SoftMax Pro MiniMax Imaging Edition

must be installed to run the

SpectraMax MiniMax Imaging Cytometer.

To prevent data loss, turn off all sleep and hibernation settings for the hard disk,
the CPU, and the USB ports. Also, disable automatic Windows Updates. You can
update Windows manually when the instrument is not being used by the software.
See

Required Computer Settings on page 17

.

Note:

Do not start the software until after the cables have been connected

and power applied to the instrument. It might be necessary to install USB
drivers before starting the software.

Содержание SpectraMax MiniMax

Страница 1: ...5024111C March 2015 SpectraMax MiniMax Imaging Cytometer Installation Guide ...

Страница 2: ...nd or license to use or permit others to use such manufacturers and or their product names as trademarks Each product is shipped with documentation stating specifications and other technical information Molecular Devices products are warranted to meet the stated specifications Molecular Devices makes no other warranties or representations express or implied including but not limited to the fitness...

Страница 3: ...ting up the cytometer includes the following steps Remove the wing nuts the transport bracket the retaining screws and the optics cover that protect the optical components during shipping See Removing the Shipping Protections on page 7 Attach the cytometer to the bottom of the microplate reader See Attaching the Imaging Cytometer to the Microplate Reader on page 9 Remove the transport locks from t...

Страница 4: ...driver slot head 5018636 SoftMax Pro Software with Product Key 5018965 USB interconnection cable 0 9 meter 3 foot 5025874 Two 2 USB computer connection cables 2 meter 6 6 foot 5022671 SpectraMax i3 Platform Transmitted Light TL Detection Cartridge 5036171 Alternative power supply The alternative power supply is required only with the SpectraMax i3 Instrument It is not required for the SpectraMax i...

Страница 5: ...B 3 0 ports and 2 or more USB 2 0 or later ports Memory 16 GB or more DDR3 SDRAM at 1600 MHz Hard disk 500 GB or larger 7200 RPM or faster hard disk with a 32 MB or more buffer Graphics display 512 MB nVIDIA graphics display 1280 x 1024 or higher resolution display Table 1 2 Minimum Computer System Requirements for the SpectraMax MiniMax Imaging Cytometer To prevent data loss turn off all sleep an...

Страница 6: ...e supplier immediately and keep the damaged packaging 2 With the box facing up as indicated on the packaging use a box cutter to carefully cut open the top of the box 3 Remove the top layer of packaging foam This piece of foam is used during the installation process 4 Remove the next layer of packaging foam that holds the tools and accessories CAUTION Keep the instrument upright and level when lif...

Страница 7: ...cure the optics plate on the top of the cytometer and a cover with three 3 blue retaining screws protects the optical components in the optics tower Item Description A Wing nuts and bolts 4 B Transport bracket and retaining screws 6 C Optics plate retaining screws 3 D Optics cover and retaining screws 3 Figure 1 2 Optics Protections for Shipping CAUTION Never touch the optic mirrors lenses filters...

Страница 8: ...lue retaining screws C CAUTION Maintain firm downward pressure on the optics plate until all the screws have been removed When the last screw is removed the optics plate might pop into position 6 Gently remove pressure from the optics plate to let the springs below the plate extend 7 Store the optics plate retaining screws in the bag that held the 3 0 mm hex key 8 Use the provided 3 0 mm hex key t...

Страница 9: ...he microplate drawer and detection cartridge drawer See the user guide for the SpectraMax i3x Instrument WARNING LIFTING HAZARD To prevent injury use a minimum of two people to lift the instrument This procedure requires the following tools and accessories Illustration Part Number Description Not applicable Foam pad from the shipping container 5024094 Hex key 2 5 mm 5024986 Laser interlock bracket...

Страница 10: ...roplate reader on its side on top of the foam pad WARNING PINCH HAZARD There is limited clearance between the side of the microplate reader and the work bench 3 Use the provided 2 5 mm hex key to remove the four screws G that secure the optics access cover F to the bottom of the microplate reader Item Description F Optics access cover G Screws 4 Figure 1 4 Optics Access Cover on the Bottom of the ...

Страница 11: ...h the brackets to secure the laser interlock bracket H to the bottom of the microplate reader 8 Attach the other laser interlock bracket H with the interlock tab I inserted into the corresponding alignment slot J and then use the other two provided screws K to secure the bracket to the bottom of the microplate reader 9 Lightly press on the interlock tabs I to make sure that they freely slide in an...

Страница 12: ...cess opening N Microplate reader feet 4 O Microplate reader foot openings 4 Figure 1 6 Attach the Cytometer to the Microplate Reader WARNING PINCH HAZARD There is limited clearance between the bottom of the microplate reader and the top of the cytometer The top of the cytometer is beveled to help provide clearance for fingers 11 Slide the cytometer into the microplate reader making sure that the o...

Страница 13: ...he threads Figure 1 7 Bolts on the Bottom of the Cytometer 13 Attach the other three bolts to secure the cytometer to the microplate reader Make sure that the bolts are properly seated on the bottom of the cytometer Figure 1 8 Properly Seated Bolt 14 Use two people to carefully stand the combined microplate reader and cytometer into an upright position 15 Store the packing materials tools optics a...

Страница 14: ...moved before powering on the instrument This procedure requires the following tool Illustration Part Number Description YW 000 006 Hex key 2 0 mm included with the microplate reader Table 1 6 Required Tool CAUTION Do not touch or loosen screws or parts other than those specifically designated in the instructions Doing so could cause misalignment and possibly void the warranty CAUTION The front cov...

Страница 15: ...wer transport lock 2 counter clockwise until it is free of the threaded hole in the floor of the drawer compartment Item Description 1 Detection cartridge drawer 2 Detection cartridge drawer transport lock Figure 1 10 Detection Cartridge Drawer Transport Lock 3 Gently slide the detection cartridge drawer 1 forward until it is outside of the main instrument 4 Lower the detection cartridge drawer tr...

Страница 16: ...awer transport lock Figure 1 11 Microplate Drawer Transport Lock 8 Use the provided 2 0 mm hex key included with the microplate reader to loosen screw 1 in the upper left corner of the transport lock 6 until the lock disconnects from the instrument frame The screw has a retaining washer that prevents it from being removed from the lock Tip After loosening screw 1 pull the microplate drawer slightl...

Страница 17: ...ftMax Pro Software requires activation The software product key is included with the software package Instructions for activating the software are included in the software installation guide and can also be found in the SoftMax Pro Software application help or user guide Microsoft Office is pre installed on the computer but requires activation The Microsoft product license is included with the com...

Страница 18: ...Imaging Cytometer requires greater minimum computer system specifications than those required for the SoftMax Pro Software See Imaging Cytometer Computer System Specifications on page 5 This procedure requires the following accessories Illustration Part Number Description 5018636 SoftMax Pro Software with Product Key 5018965 USB interconnection cable 0 9 meter 3 foot provided with the cytometer 50...

Страница 19: ...ck connect fitting 3 Power port 4 Fuse carrier 5 Power switch 6 Interconnect USB port 7 Cytometer USB port 1 8 Cytometer USB port 2 9 Alternative power input The alternative power supply is required only with the SpectraMax i3 Instrument It is not required for the SpectraMax i3x Instrument Figure 1 12 Power Switch Fuses and Connection Ports ...

Страница 20: ...sition 3 Turn on the power to the host computer 4 Install the SoftMax Pro Software on the computer See the SoftMax Pro Software installation guide or user guide The instrument is supported by SoftMax Pro Software version 6 3 or later The SoftMax Pro MiniMax Imaging Edition must be installed to run the SpectraMax MiniMax Imaging Cytometer To prevent data loss turn off all sleep and hibernation sett...

Страница 21: ...d of the USB cable to the microplate reader USB port R on the rear of the microplate reader Item Description P USB interconnection cable 0 9 meter 3 foot provided with the cytometer Q Interconnect USB port R Microplate reader USB port S USB computer connection cable 2 meter 6 6 foot provided with the cytometer T Cytometer USB port 1 U USB computer connection cable 2 meter 6 6 foot provided with th...

Страница 22: ...on for the camera It must be connected to a USB 3 0 port in the PCI card on the rear of the computer For SpectraMax I3 and MiniMax Figure 1 15 USB 3 0 Ports in the PCI Card 8 If required connect the power supply to the cytometer port on the rear of the cytometer labeled Alternative Power Input and then connect the other end of the power supply to a grounded electrical wall outlet The alternative p...

Страница 23: ...L Detection Cartridge provides white LED illumination for transmitted light imaging when used with the SpectraMax MiniMax Imaging Cytometer The StainFree Cell Detection Algorithm eliminates cell staining for cell counting and confluency measurements using proprietary transmitted light analysis technology The Transmitted Light TL Detection Cartridge occupies one 1 slot in the detection cartridge dr...

Страница 24: ...lly slide the detection cartridge drawer in or out when the instrument is powered on or when one or more detection cartridges are installed in the drawer Figure 1 16 Detection Cartridge Drawer Open 2 Select the slot or slots on the detection cartridge drawer for the detection cartridge 3 Remove the red cap from the detection cartridge before installation if supplied with the detection cartridge Th...

Страница 25: ...onto the holder pins and connector so that the detection cartridge is fully seated in the detection cartridge slot 6 Push the two retaining clips on either side of the detection cartridge so that they fasten securely to the retaining rods on both sides of the detection cartridge drawer Note Detection cartridges that occupy more than one slot have two retaining clips on each side Securely fasten al...

Страница 26: ...ng rods on both sides of the detection cartridge drawer 9 Touch the detection cartridge drawer open close button to close the detection cartridge drawer 10 Start the SoftMax Pro Software and connect to the instrument If the software is running and connected to the instrument go to the Operations tab in the ribbon and click Refresh to let the software detect the installed detection cartridges CAUTI...

Страница 27: ...escription 1 Transmitted Light TL Detection Cartridge 2 Path of white light from the Transmitted Light TL Detection Cartridge 3 Path of fluorescent excitation 4 Light source for fluorescent excitation 5 Camera lens Figure 1 19 Path of Selected Light Sources To illuminate the sample from the top you need to have the Transmitted Light TL Detection Cartridge installed in the detection cartridge drawe...

Страница 28: ...ks used herein are the property of Molecular Devices LLC or their respective owners Specifications subject to change without notice Patents www moleculardevices com productpatents FOR RESEARCH USE ONLY NOT FOR USE IN DIAGNOSTIC PROCEDURES 2015 Molecular Devices LLC All rights reserved 5024111 C ...

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