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Chapter 4: Operating the Instrument
Note: For detailed software instructions, see the
SoftMax Pro Data Acquisition and
Analysis Software User Guide
or the application help.
Temperature Settings
In the software, on the Home tab in the Ribbon, click
Temperature to display the
Temperature Control dialog. Use the Temperature Control dialog to turn the incubator on and
off and to set the chamber temperature.
You cannot set a temperature beyond the upper (45°C) or lower (4°C above ambient) instrument
limits.
Shake Settings
The software allows you to mix of the contents of the wells by shaking the plate. In the Ribbon
on the Home tab, click
Shake to shake the plate. This shake process is independent of a
protocol.
When you create a protocol, the Settings dialog allows you to define how to shake the plate as
part of the read.
For the Endpoint, Spectrum, and Well Scan read types, you can shake the plate for 0 to 999
seconds before the read at each wavelength you set.
For the Kinetic read type, you can shake the plate for 0 to 999 seconds before the initial
read, and/or before each subsequent read.
There are three shake speeds:
Low
Medium
High
There are three shake modes:
Orbital
Linear
Double Orbital
Use of shake is recommended for ELISAs and other solid-phase, enzyme-mediated reactions to
enhance accuracy.
Wavelength Settings
For absorbance reads, you use the Settings dialog to set the wavelengths. In the software,
select a Plate section in the Navigation Tree and then click
Acquisition Settings to display
the Settings dialog.
For fluorescence reads and luminescence reads, insert the filter cube that meets the read
requirements. You cannot adjust the wavelength from the Settings dialog. See
.
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