Molecular Devices QPix 450, Руководство пользователя

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QPix 450 or QPix 460 Colony Picking System User Guide
86 5031498 G
Setting Destination Plate Options
To set destination plate options:
1. In the Destination Options window, from the Select Destination Microplate list, select the
plate type to inoculate with the picked colonies.
2. Select either to dip the pins a number of times or to stir the wells of the destination plate
during inoculation.
To dip the pins a number of times, click Multi Dip and in the Number of Dips field, enter
the number of times to dip the pins.
To stir the wells, click Stir Destination.
3. Select the Make Copy check box and from the Select Copy Microplate list select the plate
type to use for a duplicate copy of the destination plate.
Note: To make a copy during the picking process, the system must have a minimum
of two available stacker lanes.
4. Select the Deposit Order to deposit the picked colonies By Columns or By Rows.
5. Define the Deposit Strategy.
Click Fill All Microplates to fill every selected well of a destination plate before starting
a new destination plate.
Click New Microplate for Each Position to start a new destination plate whenever the
instrument starts picking from a different source QTray or Petri dish.
Click New Microplate for Each Batch to start a new destination plate whenever the
instrument deck is loaded with new OmniTrays of source plates.
6. Under Destination Microplate Template, click Edit to define the wells to dip or skip. You can
skip wells that you want to use as blank or control wells. The defined template is used for all
the destination plates during the picking routine.
To skip a well, click the well. Wells to be skipped show in light blue.
To skip multiple contiguous wells, right-click and drag across the wells.
To dip a well that you skip, click the well again. Wells to be dipped show in light red.
After defining the wells to dip or skip, click Exit.
7. Click Next to select the stackers.