Molecular Devices QPix 450, Руководство пользователя

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QPix 450 or QPix 460 Colony Picking System User Guide
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3. Select the Detection tab.
4. In the Threshold intensity histogram, click and drag the red slider line to the right of the
large spike to view the White colonies as green spots. Click and drag the red slider to the
left of the large spike to view the Blue colonies as dark black spots against the background.
The X-axis represents the intensity and the Y-axis represents the relative area at that
intensity. Selecting blue or white colonies requires setting an Intensity Range.
In the Intensity Range table, select the Low and High Threshold values.
To select the Low intensity value, move the red slider line left to the lowest value, and
Ctrl+Click. The value displays in the table cell.
To select the High intensity value, move the red slider line right to the highest value,
and Ctrl+Click. The value displays in the table cell.
To manually select blue or white colonies:
To manually select Blue Colonies, select an Intensity Range that starts near the left side
of the histogram and ends to the left of the large spike that represents the largest area,
which is the agar. See Step 6 for Intensity Range setting procedures.
To manually select White Colonies, increase the minimum detectable threshold by
moving the histogram red slider line to the right. This allows the exclusion of the agar,
which is predominantly the large black area in the background, and the colonies that
are less intense than the threshold value.
5. To use Auto Select, click either Blue or White. Click the button as many times as required to
intensify the target colonies. Generally, two or three clicks get you the optimal value. Click
Reset to restore the previous intensity settings.
6. Select the Display tab.