FilterMax F3 and FilterMax F5 Multi-Mode Microplate Readers User Guide
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Item
Description
Wavelength range
(FilterMax F3)
Excitation: 340 nm to 630 nm
Emission: 390 nm to 650 nm
Wavelength range
(FilterMax F5)
Excitation: 230 nm to 650 nm
Emission: 390 nm to 700 nm
Detection limit, guaranteed
(FilterMax F3)
5 fmol/200 μL fluorescein (25 pM), black 96-well plate;
1 second integration time
2 fmol/75 μL fluorescein (26.7 pM), black 384-well plate;
400 ms integration time
Detection limit, optimized
(FilterMax F3)
3 fmol/200 μL fluorescein (15 pM), black 96-well plate;
1 second integration time
0.4 fmol/75 μL fluorescein (5.3 pM), black 384-well plate;
400 ms integration time
Detection limit, guaranteed
(FilterMax F5)
0.8 fmol/200 μL fluorescein (4 pM), black 96-well plate;
1 second integration time
0.4 fmol/75 μL fluorescein (5.3 pM), black 384-well plate;
400 ms integration time
Detection limit, optimized
(FilterMax F5)
0.3 fmol/200 μL fluorescein (1.5 pM), black 96-well plate;
1 second integration time
0.1 fmol/75 μL fluorescein (1.3 pM), black 384-well plate;
400 ms integration time
Measurement range
Up to 500 million RFU
Linear dynamic range
Up to 6 decades in a single plate read
Filter slide positions
6
Supplied wavelengths
(FilterMax F3)
Excitation: 360 nm, 485 nm
Emission: 465 nm, 535 nm
Additional filters are available.
Supplied wavelengths
(FilterMax F5)
Excitation: 485 nm, 535 nm
Emission: 535 nm, 595 nm, 625 nm
Additional filters are available.
Light source
High power LEDs
The FilterMax F5 uses a deuterium light source that extends
sensitivity into the deep UV band.
Detector
Photomultiplier tube, Single Photon Counting
Integration time per well
10 ms to 10000 ms
Table A-2: Fluorescence Intensity Top Measurement Specifications
