MOLECULAR DEVICES > CLONEPIX 2
ROBOT MANUAL
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Glossary of Terms
BARCODE
A unique label for source and destination microplates. Recommended barcoded plate types
for colonies suspended in semi-solid medium are PetriWell-1 plates (Catalogue number
W1055BC) and PetriWell-6 plates (Catalogue number W1155BC)
COLONY
A clonal group of cells, typically of mammalian origin, that have grown either adherently or
suspended in semi-solid medium.
DATUM POINT
A series of X, Y, Z co-ordinates that define a set position on the Robot bed.
DESTINATION PLATE
96-well microplate(s) prefilled with liquid medium to collect picked colonies.
DISPERSAL
Dispersal Option separates out the cells of a picked colony by aspirating and dispensing in the
destination plate.
EPI-ILLUMINATION
The white light option normally used for configuring the robot and for visualising colonies
during picking, using LEDs located under the source plate being imaged.
EQUIGLASS
A high quality polymer used as the base material for PetriWell plates. It has excellent
properties for optimal fluorescent imaging.
HALOGEN DRYER
Proprietary ultra-high temperature dryer used as part of the Pin Sanitise system.
IMAGE ACQUISITION
The capturing of images using pre-defined Acquisition Options.
IMAGE ARCHIVE
Default location for Pick Run and Imaging Run images and data. Accessible via Review
Results.
LED INTENSITY
ClonePix2 uses only LEDs for consistent and reliable imaging. The intensity of the LEDs can
be adjusted by the user to control image exposure under the Acquisition tab.
LIMS
Laboratory Information Management System.
LIVEDETECT
A reagent for visualising only live cells. Can be used as a fluorescent alternative to imaging
colonies by white light. Catalogue number K8300.
.GFP FILE
Format used to save a Process.