MOLECULAR DEVICES > CLONEPIX 2
ROBOT MANUAL
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General Maintenance
Regular Maintenance
Daily
Check that the robot interior is clean and free from any obstruction.
Ensure sterile water (feed) bottle is connected to the system fluid supply line. Empty the
waste bottle every time that a new sterile water bottle is connected.
If the system has been sanitized, purge the system fluid supply line on first use to
remove air bubbles that will have developed overnight. If there is doubt about sterility,
run the 'Prepare for Pick Run' routine which includes a purge of the system fluid supply
line with 70% ethanol or Sterilizing Agent (K8080). Sterilizing Agent (K8080) cannot be
used in North America. This should be done no more than once a week.
During the purge, check that there are no air bubbles in the supply line coming from the
sterile water bottle (indicates a fault in the connection), and that there are no leaks
around the picking head (indicates a blocked pin). If there is a leak, or if a tube detaches
from the head, replace blocked pin with a new one or follow the pin cleaning procedure
below.
Fill ethanol wash bath with 70% Ethanol. For systems fitted with a replenishing ethanol
wash bath, check that there are no kinks in the supply/waste tubing and then run
through with 70% ethanol. Check that the bath fills with ethanol and that the waste
ethanol flows freely into the waste bottle. On first use, it may be necessary to run
through several times.
Weekly
In any week that ClonePix 2 is not used, it is important to purge the system fluid supply
line with sterile water, and run 70% ethanol through the replenishing ethanol wash bath
(if fitted). Failure to keep the pumps regularly primed may lead to system malfunction.
Autoclave the picking pins.
Check operation of interlock switch and emergency stop.
Check the compressed air filter for signs of moisture. If necessary, push the drain
upwards to force moisture out.
Monthly
Sanitize picking head and silicon supply pipes (picking tubing).
Sanitize source plate illumination cover.
Annually
Preventative Maintenance Service – only to be carried out by Molecular Devices approved
personnel.
As Required
If the robot is used in particularly dusty conditions, the HEPA filter or pre-filter should be
checked and may need to be replaced more frequently than recommended (by Molecular
Devices approved personnel).
