Page 9
Ingenio® EZporator® Electroporation System
Product User Manual
| Instructions for MIR 51000
Set
-
up Instructions (Continued)
Display
Once initialized, the EZporator® Pulse Generator display will show the operational
status, voltage mode (i.e. LV or HV) and set voltage. Once a pulse is delivered to the
cuvette containing cells, the display will read the peak output voltage delivered and
the time constant.
Status options include:
Ready Mode
The EZporator® is operational and ready to deliver a pulse. The
LCD display will show HV for the high voltage mode and LV for
low voltage mode. NOTE: Ensure that the correct voltage mode
is selected for your cell type and electroporation buffer.
Use LV
mode for mammalian cell types in Ingenio® Solution.
•
LV mode: from 20 to 400 V (2 V increments), 1050 µF capacitance
•
HV mode: from 20 to 2500 V (10 V increments), 6 µF capacitance
Initializing and Charging
Initializing and charging capacitor to the preset voltage level.
Pulsing
Delivering a voltage pulse.
Feedback
Displays peak voltage and time constant for delivered pulse.
Sequence Aborted
Aborted delivery of a pulse at the user
’
s request. To abort a
pulse sequence, press the Pulse Button a second time before
delivery of the pulse.
SCR Failure
The instrument has not detected an output pulse during the
pulsing operation. As a safety precaution, this message can only
be reset by turning the power off.
Charge Failure
The capacitors failed to charge properly. To reset this message,
press the
‘
Pulse
’
button.
Setpoint Too Low
The entered voltage setpoint is below the normal operating
specifications. No pulse has been applied.
Mirus Bio
LLC
U.S. Toll Free: 844.MIRUSBIO
(844.647.8724)│
Direct: +1.608.441.2852
