Mirus Ingenio EZporator MTR 51000, Руководство пользователя

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Ingenio® EZporator® Electroporation System
Product User Manual | Instructions for MIR 51000
 Set - up Instructions (Continued)
Post - pulse Display
The EZporator® instrument will display the peak discharge voltage (V
P
) in volts (V)
and the time constant (t) in milliseconds (ms). It is normal for the measured voltage
to differ slightly from the setpoint. This is due to internal resistance and will fluctu-
ate with electrical load variations (i.e. buffer conductivity). Following the delivery of
a pulse, press the Pulse Button or rotate the Voltage Adjust knob to return to the
ready mode.
NOTE: With exponential decay generators such as the EZporator®, it is necessary to
identify and monitor the time constant (t), as this variable is dependent on the con-
ductivity and osmolarity of the electroporated sample. Significant changes in the
electroporation sample (e.g. variations in cell density, volume of nucleic acid added,
electroporation solution used) will alter the time constant.
Voltage Adjust Knob
The EZporator® Voltage Adjust knob controls set voltage AND voltage mode (i.e. LV
and HV modes). There is no separate voltage mode switch. Use the Voltage Adjust
knob to adjust the voltage from 20 to 400 V in LV mode (in 2 V increments) and from
30 to 2,500 V in HV mode (in 10 V increments). If in LV mode, increasing of the volt-
age setpoint past 400 V will result in a switch to HV mode. The voltage then read-
justs to 0 V and continues up to 2,500 V. If in HV mode, decreasing the voltage set-
point past 0 V will result in a switch to LV mode. The voltage then readjusts to 400 V.
NOTE 1: The EZporator® emits a sound when switching between LV and HV modes.
NOTE 2: The Voltage Adjust knob is speed sensitive. A quick rotation increases the
rate of change of the voltage setpoint.
NOTE 3: LV mode is used for mammalian and insect cell types.
Pulse Button
The Pulse button is activated in ‘ READY ’ mode. Once pressed, the generator will
bleed off the capacitors to the preset voltage, prior to delivering the pulse. The
capacitor bleed time is typically between 1
- 6 seconds. A pulse sequence may be
aborted by pressing the Pulse button a second time before delivery of the pulse.
Following the delivery of a pulse, press the Pulse button or rotate the Voltage Adjust
knob to return to the
‘ READY ’ mode.
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