Mirus Ingenio EZporator MTR 51000, Руководство пользователя

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Ingenio® EZporator® Electroporation System
Product User Manual | Instructions for MIR 51000
 Technology Overview
Electroporation is a physical transfection method that utilizes short electrical pulses
to create transient membrane pores in cells through which small particles (e.g. DNA,
RNA, proteins, etc.) can pass. Optimal electroporator pulse settings and composition
of cell resuspension solution greatly enhance electro
- permeabilization and promote
passage of particles through the cell membrane. Since electroporation is an effective
transfection method regardless of the type of molecule delivered or the target cells,
this technique provides a robust and universal approach for multiple applications,
including gene expression, RNAi, stable cell line generation and CRISPR/Cas genome
editing.
Electroporation is an invaluable alternative for cell types that are not responsive to
chemical transfection and often yields significantly higher transfection efficiency.
Optimizing parameters such as nucleic acid amount, cell density and voltage for
each cell type will improve transfection efficiency and decrease cell mortality, thus
making electroporation suitable for transfecting various cell types with any type of
nucleic acid or small molecule.
The Ingenio® EZporator® Electroporation System was developed with both efficiency
and simplicity for the researcher in mind. This system utilizes an
exponential decay
pulse * to effectively deliver cargo to cells in a cuvette with the push of a button. The
Ingenio® EZporator® was designed for use with Ingenio® Electroporation Kits and
Solution. Use of electroporation solutions not manufactured by Mirus Bio may result
in low transfection efficiency and high cellular cytotoxicity. Additionally, use of in-
compatible electroporation solutions may cause damage to the instrument and will
void the warranty. More information on Ingenio® Electroporation Solution and Kits
is available at
mirusbio.com/ingenio .
*In exponential - decay pulse conditions, a set voltage is released from the capacitor and decays
exponentially. See Optimization Section (page 14) for additional details.
Ingenio® EZporator® Electroporation System and Ingenio®
Electroporation Solution, cuvettes and cell droppers.