Miltenyi Biotec UltraMicroscope II, Руководство пользователя

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7.3 Capturing a multi-color z-stack
1 Select Measurement Mode > Multi Color Acquisition in the Measurement Wizard window.
2 Select xyz-Table Z and UltraII Filter in the Devices list.
3 Click the Autosave button at the end of the row under AS . It will turn green.
4 Select the correct excitation and emission wavelengths for your sample under Filter for Measurement in
the Settings 1 window. The selected checkboxes are used.
5 Move the slider under Laser Transmission Control while the filter is highlighted to set the laser power for
each individual filter.
NOTICE! Higher laser power will bleach the sample.
6 Select the shortest emission wavelength, and create a sharp picture.
7 Correct the different focus lengths for different emission wavelengths using the Chromatic Correction
button, starting with the next longer wavelength.
8 If all emission wavelengths have been corrected, click Save Settings in the toolbar.
9 To start the measurement, proceed as described under Capturing an image on the previous page .