Miltenyi Biotec UltraMicroscope II, Руководство пользователя

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4 Check by eye that the alignment tool is in a centered position, and move it into the light sheets by using
the jog wheel. The light sheets from the right-hand side should pass the pinhole and form thin lines on the
other side. The sharper the lines, the better is the z-position.
5 Decrease the sheet width to the standard, refer to Setting the parameters for the alignment on
page 26 .
6 Lower the objective lens, and focus on the light sheets.
7 Click the Show crosshair button on the right side of the live window to activate the crosshair.
8 Place the Rayleigh length in the center of the field of view by clicking the Rayleigh ROI button under the
Measurement tab in the Settings 1 window.