3.2 Capillary connections in the IC system
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16
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940 Professional IC Vario TWO (2.940.2100)
occurs if two capillary ends connected to each other do not fit exactly,
thus allowing liquid to escape. There are two possible causes for this:
￭
The capillary ends do not have exactly flat edges.
￭
The two capillary ends do not completely meet.
One prerequisite for dead-volume-free capillary connection is that both
capillary ends are cut exactly flat. Therefore we recommend cutting PEEK
capillaries only with a capillary cutter (6.2621.080).
Also see:
Cutting capillaries
Creating dead-volume-free capillary connections
To create dead-volume-free capillary connections, proceed as follows:
1
Wipe off the end of the capillary with a cloth dampened with ace-
tone.
2
Slide the pressure screw over the capillary. Ensure that the capillary
protrudes 1 to 2 mm from the tip of the pressure screw.
3
Push the capillary into the connection or coupling as far as it will go
and hold it there.
4
Only then start turning the pressure screw. Hold the capillary in the
stop position while turning it shut.
Colored sleeves for PEEK capillaries
The enclosed set of varicolored sleeves for PEEK capillaries (6.2251.000)
serves to easily differentiate the various flows of liquid in the system
through color coding. Each capillary conveying a given liquid (e.g. eluent)
can be marked with sleeves of the same color.
1
Slide a colored sleeve of a selected color over a capillary and move it
to an easily visible position.
2
Heat the colored sleeve, e.g. with a hairdryer.
The colored sleeve shrinks and adapts to the shape of the capillary.
NOTE
In order to arrange capillaries more clearly, they can be bundled with
the spiral band (6.1815.010).
