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Introduction to this manual

Chapter 1

Zetasizer APS Basic Guide

Page 1-3

Contact the United Kingdom help desk if the local Malvern representative is not
available. The direct line to the United Kingdom Helpdesk is +44 (0) 1684 891800.
This help line is primarily English speaking.

Remote support

Malvern Instruments offers a remote support service over the Internet. (A direct
Internet connection must be available.)

Benefits include fast and efficient fault diagnosis, reducing downtime and costs.
Online user training is also available, plus software updates.

Malvern website - www.malvern.com

The Malvern website offers a comprehensive range of particle characterization
resources for use by customers 24 hours a day, seven days a week.

Resources include software downloads, frequently asked questions, a knowledge
base and

Application Notes

, plus information on other particle characterization

solutions that Malvern can provide.

MAN0494-1 (Zetasizer APS Basic Guide).book Page 3 Friday, August 9, 2013 2:08 PM

Содержание APS5000

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Страница 3: ...Zetasizer APS Basic Guide MAN0494 Issue 1 0 July 2013 English MAN0494 1 Zetasizer APS Basic Guide book Page i Friday August 9 2013 2 08 PM...

Страница 4: ...al No reproduction or transmission of any part of this publication is allowed without the express written permission of Malvern Instruments Ltd Head office Malvern Instruments Ltd Enigma Business Park...

Страница 5: ...2 5 Health and safety General warnings and Safety regulations 3 1 Electrical warnings and Safety regulations 3 2 PAT testing 3 2 Power cords and Power safety 3 2 Laser safety regulations 3 4 Temperat...

Страница 6: ...3 Replacing the system fuse 5 8 Replacing the tubing and needle 5 8 Installing the Zetasizer APS 5 19 Changing the computer 5 21 Appendix Specification A 1 Chemical compatibility A 2 Disposal of Elect...

Страница 7: ...ftware use its Help system Each window has a Help button giving information about it Advice buttons present sample related content Menu commands Software menu commands are referred to in the form main...

Страница 8: ...and safety of the instrument and its operation The supervisor also trains the operators They can perform all user maintenance routines identified in Chapter 5 The supervisor has access to a more deta...

Страница 9: ...be available Benefits include fast and efficient fault diagnosis reducing downtime and costs Online user training is also available plus software updates Malvern website www malvern com The Malvern we...

Страница 10: ...Chapter 1 Introduction to this manual Page 1 4 MAN 0494 MAN0494 1 Zetasizer APS Basic Guide book Page 4 Friday August 9 2013 2 08 PM...

Страница 11: ...ument is delivered Environmental conditions The site must be Indoors and away from strong light windows Away from heat sources like radiators Well ventilated for noxious materials On a horizontal vibr...

Страница 12: ...m the left side of the PTC 1000 and this will prevent hot air being directed towards the APS ill 8330 Allow at least 800mm above the bench surface for access to the plate holder area The system dimens...

Страница 13: ...with this product on any other products Power cord set requirements Power cord sets must meet the requirements of the country where the product is used For more information contact a Malvern represent...

Страница 14: ...ontact the Malvern Helpdesk or website for the recommended computer specifi cation otherwise consult the Software update notification document supplied on the software CD Note A laptop computer must h...

Страница 15: ...nstru ment and invalidate the warranty Telephone socket specification The telephone socket for remote support needs a direct Internet connection Laser safety Zetasizer APS instruments are Class 1 lase...

Страница 16: ...Chapter 2 Site requirements Page 2 6 MAN 0494 MAN0494 1 Zetasizer APS Basic Guide book Page 6 Friday August 9 2013 2 08 PM...

Страница 17: ...protection provided by the system The instrument must only be stored or operated in environmental conditions con forming to Chapter 2 Positioning the Instrument Warning Do not position the instrument...

Страница 18: ...plying power Warning This product must be connected to a protective earth The metal parts of the optical unit and the accessories are earthed via a protective earth connection PAT testing If PAT testi...

Страница 19: ...0 75mm2 or 18AWG and the length of the cord must be less than 3m The power cord must be routed so it will not be walked on pinched by items placed on or against it or become wet Pay particular attent...

Страница 20: ...ers when used Warning Do not use adapter plugs that bypass the grounding feature or remove the grounding feature from the plug or adapter Place the AC adapter in a ventilated area such as a desk top o...

Страница 21: ...in hazardous exposure to laser radiation This diagram shows the location of the laser warning labels ill 8768 Temperature warnings Warning The temperature range of the measurement area is 2 C to 90 C...

Страница 22: ...ound caused by broken glass Always test a new sample or solvent for chemical compatibility before use After measuring hazardous samples scrupulously clean the system to remove any contaminants before...

Страница 23: ...mpting to move the system Always adopt proper lifting techniques to avoid back injury Always lift the instrument by holding the under its base Never lift an instru ment by its covers Refer to the Unpa...

Страница 24: ...accordance with the European Disposal of Electrical Electronic Equipment regulations ill 7610 This symbol on the product or on its packaging indicates that when the last user wishes to discard this p...

Страница 25: ...measurements refer to the Zeta sizer APS User Manual and software help system Switching on starting the software 1 Turn on the instrument by pressing the power switch on the back of the instrument 2 D...

Страница 26: ...hen run a measurement by using a plate schedule and an SOP 1 Load a plate with sample Prepare a sample for example 60nm latex or BSA 2 3 mg ml Fill the wells with a minimum sample volume of 20 L for a...

Страница 27: ...menu bar The Plate Scheduler is displayed 4 Set up the Schedule Settings 1 Click the Schedule Settings button on the lower toolbar to display the Schedule Settings window 2 Enter any identifying text...

Страница 28: ...eneral purpose sop from the Protein folder 2 Select the well s containing the sample by clicking on them A red outline shows that the well is selected 3 Click on the name of the SOP in the left panel...

Страница 29: ...the plate is oriented correctly in the plate holder with the top left of the plate i e the origin of the plate s well identification markings closest to the instrument This is critical and ensures tha...

Страница 30: ...er a file name and click Save 3 The plate holder withdraws into the instrument and the measurement process begins As the SOP analysis runs the results are displayed 4 Further messages in the status ba...

Страница 31: ...results are stored as the latest record in the currently open measurement file To view the result select this record and then click one of the Report tabs The following example shows the Aggregation...

Страница 32: ...Chapter 4 Making a measurement Page 4 8 MAN 0494 MAN0494 1 Zetasizer APS Basic Guide book Page 8 Friday August 9 2013 2 08 PM...

Страница 33: ...er maintenance procedures that can be performed These procedures are Removing or replacing the transit locks Cleaning the instrument Replacing the system fuse Cleaning and replacement of the tubing ne...

Страница 34: ...to the centre of the tray by applying light finger pressure 3 Switch on the instrument and then start the software The needle will move to the wash station 4 Restart the software to initialise the nee...

Страница 35: ...d practice never to use a sol vent based solution as this may damage the painted surfaces Never use an abrasive cleaner to clean the instrument as this may damage the painted surfaces Never use compre...

Страница 36: ...L of DI water Step 2 3750 uL of 0 1M NaOH Step 3 5000uL of DI water Custom wash Where the volumes for the rinse and wash fluids can be set as a rinse protocol recommendations and examples are describe...

Страница 37: ...1250 l of wash fluid and finally 5000 l of rinse fluid Deionised water is suggested as the rinse fluid and 0 1M NaOH as the wash fluid For thermal trend measurements Use either use vigorous wash or s...

Страница 38: ...the instrument cleanliness Note It is recommended that a stable count rate no greater than 500kcps is achieved before any measurements are performed The cleanliness of the instrument can be monitored...

Страница 39: ...count rate will be displayed With a clean instrument you should see no correlation and the count rate should be more or less a straight line with random fluctuations of less than 10kcps The count rat...

Страница 40: ...Replacing the tubing and needle General safety Warning Before replacing parts always disconnect the instrument from the power supply and disconnect all electrical cables Warning A small volume of res...

Страница 41: ...asure ment block Rinse tubes and ferrule connectors Rinse inlet filters and ferrule connectors Measurement block flowcell The following parts can be ordered from your local Malvern Instruments distrib...

Страница 42: ...f the system remove the cover by lift ing it vertically off the instrument as shown in the illustration below ill 8583 On removing the lid a laser safety interlock is activated which prevents the mach...

Страница 43: ...older Rinse well Syringe pump Well plate holder shown with plate in situ Measurement block tubing and connections The tubing and respective connection to the measurement block are manufac tured as one...

Страница 44: ...ng Follow the process below Note The measurement block flowcell is a critical optical component Utmost care must be taken on all aspects of the cleaning procedure 1 Unscrew the sample needle bottom co...

Страница 45: ...build up is suspected inside the flowcell a wetted interdental brush can be used to gently clean the internal channel Take great care when per forming this operation as the central core of the brush...

Страница 46: ...r a reasonably clean system this should be less than 50kcps If this is not the case check the con nections the cleanliness of the wash fluid and finally repeat the cleaning of the flow cell itself Ref...

Страница 47: ...be is clamped securely Rinse inlet filters The two rinse inlet filters are of microporous structure to prevent foreign particles entering the system and causing inaccurate measurements Both external r...

Страница 48: ...hat is currently being used The piercing needle included is more suited to piercing the membrane used to cover some plates whereas a flat tipped needle included could be more effective in plates with...

Страница 49: ...s described When seating the needle in the needle holder ensure that the exposed needle length is precisely 50mm 1mm Failure to do this could result in damage to the needle Do not over tighten the sec...

Страница 50: ...For this section please refer to the illustrations earlier in this chapter There are three rinse tubes inside the machine and two located externally ill 8585 IMAGE TO BE UPDATED A C B A B A E D C MAN0...

Страница 51: ...may be company policy to periodically upgrade computers Detail is given on actions required Only install the system in a suitable environment as detailed in Site Requirements section of this document...

Страница 52: ...the machine is shipped to another destination ill 8771 Connect the USB cable to the connector marked USB on the rear panel of the Zetasizer APS instrument Connect the other end of the cable to the US...

Страница 53: ...perature Control unit connect to input Changing the computer If at some point in the future the computer used with the instrument is changed the following actions will be required To install the syste...

Страница 54: ...Chapter 5 Maintenance Page 5 22 MAN 0494 MAN0494 1 Zetasizer APS Basic Guide book Page 22 Friday August 9 2013 2 08 PM...

Страница 55: ...2 C 2 C 0 5 C 90 C Weight 22 5kg Dimensions W D H 500mm x 410mm x 420mm Power 90V 260V AC 50 60Hz 150W Dry air 100kPa 1bar 4mm o d tubing Computer interface USB Measurement technique Dynamic light sca...

Страница 56: ...emi cally compatible with the materials mentioned Warning It is advisable that the chemical compatibility is checked against the materi als identified below before inserting a sample It is also recomm...

Страница 57: ...t when the last user wishes to discard this product it must not be treated as general waste Instead it shall be handed over to the appropriate facility for the recovery and recycling of electrical and...

Страница 58: ...y not cause harmful interference and 2 this device must accept any interference received including interference that may cause undesired operation Note This equipment has been tested and found to comp...

Страница 59: ...uipment This Class A digital apparatus complies with Canadian ICES 003 Cet appareil num rique de la classe A est conforme la norme NMB 003 du Canada VCCI acceptance Japan only The Voluntary Control Co...

Страница 60: ...Appendix A Appendix Page A 6 MAN 0494 MAN0494 1 Zetasizer APS Basic Guide book Page 6 Friday August 9 2013 2 08 PM...

Страница 61: ......

Страница 62: ...www malvern com Malvern Instruments Limited Grovewood Road Malvern Worcestershire WR14 1XZ UK Tel 44 1684 892456 Fax 44 1684 892789...

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