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Image-based: Make sure the current field is focused sharply. Set the range to scan
from the current focal position. You can choose to turn the user-defined focus offset
on or off. The user-defined focus offset means that the system will calculate the
difference between what the system defines as the optimal focal plane and what the
user defines as the focal plane of interest and automatically calibrate the focus
accordingly.
Laser AF: Make sure the current field is focused sharply. The vessel information
must be correct. Click Pre-Scan to have the CELENA® X configure the laser
autofocus settings. Click Test AF to test the accuracy of the configured laser
autofocus. Laser AF cannot be used with magnifications below 10X.
Add to the project protocol.
Click Add.