14 – LABNET BIOPETTE PLUS PIPETTORS
Filters
The 5,000 μL and 10,000 μL pipettors include a replaceable
filter (L), fitted at the bottom of the shaft which
prevents aspirated liquid from entering the shaft and
contaminating the inner surface and plunger. Use of
the filter is particularly important when aspirating and
dispensing large volumes of liquid. The filter should be
replaced if it becomes wet.
9. Checking Pipetting Accuracy Parameters
and Pipettor Recalibration
The pipettors have been factory-calibrated using
gravimetric methods with manufacturer pipet tips and
distilled water, in accordance with ISO 8655 guidelines
for the maximum (nominal) liquid volume drawn by the
pipettor and for 10% of the maximum or minimum liquid
volume according to the values given (Section 5).
The pipettors are designed to enable recalibration and
adaptation to different pipetting techniques and liquid
properties (e.g., temperature, density, and viscosity).
Periodic checks of the operation of the pipettor are
recommended at least once per year. Frequency of checks
should be increased depending on workload, sterilization
or autoclave processes, and frequency of replacement of
component parts.
If during pipettor operation the accuracy error (the
difference between the real aspirated volume and the
preset volume) exceeds the permissible value given
in the table in Section 5, pipettor recalibration should be
carried out.
Recalibration of the pipettor involves volume adjustment
based on the value obtained by weighing. Recalibration of
the pipettor is performed only for one liquid volume drawn
by the pipettor.
The recommended volume for recalibration is 10% of the
maximum (nominal) value or minimum volume depending
on which of these values is greater. See table on page 16 for
more details.