Knauer Azura ECD 2.1, Инструкция по эксплуатации

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22 Installation
AZURA® Detector ECD 2.1 Instructions, V6685
Legend
1 Cell clamp
2 Cell Inlet (tubing connec-
tion from column–to–cell)
3 Cell outlet (tubing
connection from cell-to-
waste)
4 WE contact (red)
5 AUX contact (blue)
6
REF contact (black)
Fig. 14 Left: SenCell with ISAAC reference mounted under an angle of
approximately 45° in the detector. Make sure that the outlet [3] is
positioned on the top side to prevent entrapped air bubbles.
Top-right: electrical connections of WE (red connector) and AUX elec-
trode (blue connector).
Bottom-right: SenCell with SaltBridge reference electrode.
9. Before switching ON the flow cell, make sure that the mobile phase
contains sufficient electrolyte (buffer ions). A stable baseline will never
be obtained if the cell is switched ON with only water or another
non-conducting mobile phase. Also be sure that no air bubbles are
trapped in the flow cell.
10. The outlet tubing from the flow cell should lead to a reservoir that is
at a higher level than the flow cell. This ensures a small back pressure
which prevents air-bubble entrapment. The outlet tubing should be
under the liquid level, to avoid electrical noise induced by ‘dripping’
of mobile phase.
11. Set the cell potential (see page 54 for optimization of the poten-
tial), switch ON the flow cell and allow the system to stabilize for
approximately 30 min. A ‘good’ stabilization curve shows a mono-ex-
ponential decline without jumps and/or spikes.
12. Connect the ECD 2.1 to the PC control software (ClarityChrom® or Eli-
te Dialogue). In the case the instrument is used stand-alone connect
an external A/D converter or recorder to the ‘Analog data output’
(1 V full scale) on the rear panel. Refer to the documentation of the
third-party software controlling the A/D converter for detailed instruc-
tion how to set up a measurement with the A/D device.
Your system is now ready for use. The ECD 2.1 has been developed for
continuous operation. For maximum stability it is advised to leave the
system ON continuously. If preferred, the flow cell may be switched OFF
at night.