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Kendro SORVALL Biofuge primo, Руководство по эксплуатации

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Operation 

  33

5. For faster operation, you may shift the flashing 

cursor in the speed/RCF and in the run time panels: 
just press both 

 and 

 simultaneously. The cur-

sor moves by one digit to the left for each key 
depression. 

Entering the RCF value 

You can adjust the RCF setpoint in steps of 1. The 
setpoint is entered analogously to the speed. 
As long as the rotor has not been identified, it is im-
possible to display RCF values. This is signaled by 
dashes ----- in the display. 
Shortly after starting the centrifuge run the rotor is 
identified, and the current value is displayed. 

NOTE: 

If you set an extremely low RCF value, this may be 
automatically corrected if the resulting speed would be 
lower than 300 rpm. 

Concerning the RCF value  

The 

r

elative 

c

entrifugal 

f

orce (RCF) is given in multi-

ples of the earth gravity 

g

. It is a dimensionless num-

ber that allows one to compare the efficiency of sepa-
ration or sedimentation of diverse instruments, since it 
is independent of the instrument used. The only values 
entered in the equation are radius and speed of cen-
trifugation: 

RCF

n

r

=

∗ 





∗

1118

1000

2

.

 

r = radius of centrifugation in cm 
n = speed in rpm 

 

The maximum RCF value is based on the maximum 
radius of the vessel bore. 

 

Please note that this value becomes lower 
depending on the tubes and adapters used. 

 

 

You may take this into account when calculating the 
RCF value for your application. 

Содержание SORVALL Biofuge primo

Страница 1: ...SORVALL Biofuge primo OPERATING INSTRUCTIONS PN 00000 0...

Страница 2: ...nnot provide protection against improper handling and thus against damage to persons and objects The manual comprises chapters on Safety regulations Instrument description Rotor program and accessorie...

Страница 3: ...back panel socket for mains cable mains switch rpm RCF display switch set keys quick run open lid stop start speed RCF run time braking profiles...

Страница 4: ...open lid possible only with the instru ment switched on Quick run short term operation of the centrifuge as long as key remains pressed rpm RCF switch Switching between rpm and RCF dis play Set keys s...

Страница 5: ...ors 21 Swinging Bucket Rotors 21 Lifetime of the rotor 21 Permissible rotor temperature 21 Highconic rotor 22 Drum rotor 22 Aerosol tight operation 23 Mikroliter rotor 75007587 and PCR rotor 75007569...

Страница 6: ...trifugation 36 Removing the rotor 37 Maintenance and care 39 Maintenance to be performed by the customer 39 Cleaning 39 Disinfection 40 Decontamination 42 Autoclaving 43 The KENDRO service offer 44 Wa...

Страница 7: ...at all times When damages at the power cord or at casing were noticed the centrifuge has to be set out of operation Proper use The centrifuge is designed to separate liquid suspended materials having...

Страница 8: ...l accessories for the centrifuge The only exceptions are common glass or plastic centrifuge tubes if they are approved for the rotor speed and RCF values Never use the centrifuge unless the rotor is p...

Страница 9: ...m the technical data Safety instructions in this manual This symbol denotes potential hazards to persons This symbol denotes potential damage to the centrifuge or parts in its immediate surroundings G...

Страница 10: ...For your safety 6 Notes...

Страница 11: ...d only when the power is turned on and the rotor has come to a halt You can start the centrifuge only if the lid is properly locked Rotor identification for power adjustment Electronic unbalance detec...

Страница 12: ...The Biofuge primo 8 for your notes...

Страница 13: ...ection of speed RCF value run time and run profile acceleration and braking behavior You can switch from speed to RCF display or entry and vice versa You can change the set values even during a run Wi...

Страница 14: ...y board key and display elements covered by an easy care protective foil control microprocessor driven by Easycontrol II program memory the data last entered remain in memory functions RCF preselectio...

Страница 15: ...ease acceleration braking profiles 1 slow acceleration and slowest braking curve 2 2 9 fast acceleration and 8 different braking profiles speed selection adjustable in steps of 10 min 1 within the ran...

Страница 16: ...The Biofuge primo 12 Function Feature diagnostic messages incorrectly closed lid display OPEN general malfunction error messages ERROR codes see chapter Troubleshooting...

Страница 17: ...meet the following criteria A safety zone of at least 30 cm around the centri fuge must be maintained where neither persons nor hazardous materials may be stationed during cen trifugation The substru...

Страница 18: ...pond to the specifications on the instrument label Turn the mains switch on the back panel off press 0 only then connect the centrifuge with the mains supply via the power cord mains switch type plate...

Страница 19: ...s see Rotor program Table 1 In addition there are sets of adapters and reduction sleeves for diverse commercially available vessels see Adapters Table 2 Please consult our sales documentation for a co...

Страница 20: ...le load g 6 x 130 4 x 200 8 x 80 maximum speed nmax min 1 8500 4000 12 000 maximum RCF value at nmax 10 016 2522 14 005 maximum radius cm 12 4 14 1 8 7 minimum radius cm 6 0 5 0 3 8 angle 45 90 90 60...

Страница 21: ...aximum RCF value at nmax 21 882 16 060 12 846 11 524 2 maximum radius cm 8 7 8 5 6 8 6 1 2 minimum radius cm 5 9 5 9 6 1 4 7 2 angle 45 40 45 acceleration time s 40 15 15 braking time s 30 25 25 aeros...

Страница 22: ...12 ml 16 x 95 2 12 7500 3093 16 ml 18 x 122 1 6 7600 2906 38 ml 25 x 112 1 6 7500 3094 50 ml 29 x 122 1 6 7500 3014 15 ml Falcon 16 5 x 120 1 6 7500 3095 50 ml Falcon 30 x 117 1 6 7500 3096 Adapters f...

Страница 23: ...ml DIN 1818 12 x 102 5 20 white 7500 7545 7 ml DIN blood sampling 1818 13 x 105 3 12 white 7500 7546 15 ml DIN blood sampling 1803 17 x 102 3 12 white 7500 7544 25 ml DIN 1804 25 x 110 1 4 white 7500...

Страница 24: ...tor color order no 1 5 ml microvessels 10 80 yellow 7600 1499 1 5 2 ml microvessels 10 80 red 7600 1244 1 5 2 ml microvessels 60 6 48 white 7500 1498 0 3 ml microcapillary vessels 8 64 blue 7600 1246...

Страница 25: ...e buckets Lubricant 7000 6692 is supplied with the centrifuge Lifetime of the rotor There is no limitation on the service life of the high performance rotors However please observe the fol lowing due...

Страница 26: ...ged precluding the complete locking of the rotor lid it is allowed to operate the rotor without lid up to a maximum speed of 4000 rpm Above 4000 rpm the rotor may not be operated without lid in any ca...

Страница 27: ...ion or damage and slightly greased if necessary Replace damaged O rings and seals For greasing the seals only use the special lubricant 7600 3500 Replacements parts are delivered with the rotor or can...

Страница 28: ...tor Insert the O ring into the inner groove on the screw on top Loading for aerosol tight operation For the centrifugation of hazardous samples you must always respect the highest permissible sample a...

Страница 29: ...0 ml 1 5 ml microliter rotor 24 x 2 0 ml 75007587 Reakt 1 5 ml 1 0 ml Reakt 2 0 ml 1 5 ml Highconic rotor 6 x 50 ml 75007588 Falcon 50 ml 49 ml Falcon 15 ml 14 ml swinging bucket rotor 75007591 Glas...

Страница 30: ...sted for wear and damages like cracks scratches and embrit tlement carefully As a quick test there is the possibility to check the aerosol tight buckets and fixed angle rotors according to the followi...

Страница 31: ...for the remaining run time and speed both read 0 default value is speed The display of the acceleration braking curve depends on the value last set The following figure gives an example of possible re...

Страница 32: ...ms delivered Possible damage to drive and rotor You may insert the rotor only if the temperature of the drive the rotor and the cap nut is between 10 C and 30 C Proceed as follows 1 Open the lid and m...

Страница 33: ...the cap nut easily and secure it with the tubular socket wrench delivered with the instru ment Do not push the rotor down using force If you cannot screw on the cap nut you must carefully lift off the...

Страница 34: ...ssible speed nperm according to the following for mula n perm n max maximum permissible load actual load Filling the centrifuge tubes Please note that plastic sample vessels only have a limited servic...

Страница 35: ...3 4 5 6 7 8 9 1 0 1 1 1 2 1 3 1 4 1 5 1 6 1 7 1 8 1 9 2 0 2 1 2 2 23 24 1 2 3 4 5 6 7 8 9 1 0 1 1 1 2 1 3 1 4 1 5 1 6 1 7 1 8 1 9 2 0 2 1 2 2 23 24 max 13000 r p m H E R A E U S I N S T R U M E N T S...

Страница 36: ...d and RCF entry or display Selecting the speed The centrifuge can be set to a minimum of 300 and a maximum of 15 000 min 1 depending on the rotor You can adjust the speed in steps of 10 min 1 Proceed...

Страница 37: ...n extremely low RCF value this may be automatically corrected if the resulting speed would be lower than 300 rpm Concerning the RCF value The relative centrifugal force RCF is given in multi ples of t...

Страница 38: ...ou keep the selected key pressed the display changes at first slowly and after a few seconds at an accelerated pace 4 Release the key as soon as you have reached the desired value and fine tune if nec...

Страница 39: ...ssible value for the inserted rotor Within 15 seconds you may adopt this value by again pressing the start key the centrifugation is then con tinued Otherwise the centrifuge stops and you must enter a...

Страница 40: ...top key in the control panel The centrifuge starts braking with the preset braking profile The display reads End and you can open the lid by pressing the open lid key and remove your samples Short tim...

Страница 41: ...e centrifuge lid 2 Remove the rotor lid 3 Unscrew the cap nut by turning it counterclockwise using the socket wrench supplied and remove the cap nut 4 Grab the rotor with both hands and lift it carefu...

Страница 42: ...Operation 38 for your notes...

Страница 43: ...ake sure by consulting the manufacturer that the procedure foreseen does not cause any dam ages to the instrument Cleaning Pull mains plug before cleaning the instrument Clean the casing the rotor cha...

Страница 44: ...ng a run you have to disinfect the centrifuge im mediately Infectious material could enter the centri fuge if spills or tube breakage occur Danger of infection may occur upon con tact Take appropriate...

Страница 45: ...nent safety procedures for handling infec tious material 1 Pull mains plug 2 Unscrew the rotor chuck 3 Grab the rotor with both hands and pull it perpen dicularly off the drive shaft 4 Remove the cent...

Страница 46: ...and the rotor should not be warmer than ca 25 C 2 Do not let the bleaching solution act longer than absolutely necessary 3 After disinfection rinse the rotor thoroughly with distilled water and allow...

Страница 47: ...87 and 75007569 maximally 10 times The rotor must be cleaned and rinsed with distilled water before being autoclaved Remove the rotor lid the centrifuge tubes and the adapters Place plastic rotors on...

Страница 48: ...ering it Inspection costs are charged as flat rate con tracts Necessary repairs are carried out free of cost within the warranty conditions and against payment after expiration of the warranty period...

Страница 49: ...ing it may cause severe injuries Always wait for several minutes until the rotor has come to a complete stop Without power the brake does not function and braking takes much longer than normal Proceed...

Страница 50: ...urns close lid immediately and wait until it has come to a complete stop 5 As soon as the rotor stands still remove your samples and close the lid Finally push the rip cord back into the instrument an...

Страница 51: ...stops The rotor stops without braking The lid cannot be opened Mains failure or not connected 1 Is the mains plug connected to the mains socket 2 Check the mains connection 3 If the mains connection...

Страница 52: ...this is unsuccessful you may open the lid using the emer gency lid release see page 45 B Heat monitoring relays of the lid unlocking mechanism have been actuated Press key again after a short pause a...

Страница 53: ...ge rotor appears in dis play Rotor decelerates with brake on Set speed exceeds permissible maximum speed for the rotor in question The same holds for RCF setting A For about 15 sec the display shows a...

Страница 54: ...nt after 20 min If the safety circuit is triggered again you must call our Service Message Lid appears in the display Drive stops Rotor coasts to rest A Lid was opened manually during the run 1 Press...

Страница 55: ...off and on again If the error persists call our Service E 03 Rotor stops without brak ing to standstill Instrument cannot be operated Error in speed measurement Switch the instrument off and on again...

Страница 56: ...a rotor is inserted B Following a brief power failure the rotor could not be identified Switch the instrument off and on again using the mains switch C Phases at the motor mixed up E 15 Rotor stops w...

Страница 57: ...without brak ing to standstill Start without rotor 1 Turn instrument off and on again 2 Open the instrument by pressing the Open lid key 3 Check whether the rotor is loaded and placed correctly 4 Chec...

Страница 58: ...ion as follows 1 Switch the instrument off 2 Switch the instrument on All displays read 8 88888 for about one second Subsequently the display may read e g the follow ing readings for 2 seconds each So...

Страница 59: ...rature of the environment 2 C to 40 C run time 1 min 9 h 59 min hold permanent operation maximum speed nmax 15 000 min 1 rotor dependent adjustable in steps of 10 minimum speed nmin 300 min 1 maximum...

Страница 60: ...CSA C22 2 No 1010 1 92 CAN CSA C22 2 No 1010 1 B97 amendment 2 UL 3101 1 pending EN 292 EN 61 010 1 EN 61 010 2 020 EN 61326 EN 55011 B Electrical connections fuses Order no Voltage Frequency Nominal...

Страница 61: ...Appendix 57 Appendix...

Страница 62: ...Appendix 58 Braking and acceleration curves Acceleration curves time s 4000 3000 2000 1000 0 0 20 40 60 80 100 1 9 2 speed min 1...

Страница 63: ...Appendix 59 Braking curves 4000 3000 2000 1000 0 0 50 100 150 2 1 3 4 5 9 8 6 7 speed min 1 time s...

Страница 64: ...Appendix 60...

Страница 65: ...Appendix 61 Speed RCF diagrams 75007588 Highconic rotor 6 x 50 ml 1 10 100 1000 10000 100000 100 1000 10000 100000 RCF rmax 12 4 cm RCF rmin 6 0 cm nmax 8500 min 1 RCF rmax nmax 10016 RCF speed min 1...

Страница 66: ...Appendix 62 1 10 100 1000 10000 100 1000 10000 75007591 swinging bucket rotor 4 x 100 ml RCF rmax 14 5 cm RCF rmin 5 0 cm nmax 4000 min 1 RCF rmax nmax 2522 RCF speed min 1...

Страница 67: ...Appendix 63 1 10 100 1000 10000 100000 100 1000 10000 100000 75007593 fixed angle rotor 24 x 1 5 2 ml RCF speed min 1 RCF rmax 8 7 cm RCF rmin 5 9 cm nmax 17000 min 1 RCF rmax nmax 28106...

Страница 68: ...Appendix 64 1 10 100 1000 10000 100000 100 1000 10000 100000 75007587 microliter rotor 24 x 1 5 2 ml RCF rmax 8 5 cm RCF rmin 5 9 cm nmax 13000 min 1 RCF rmax nmax 16058 RCF speed min 1...

Страница 69: ...Appendix 65 1 10 100 1000 10000 100000 100 1000 10000 100000 75007569 PCR rotor RCF rmax 8 5 cm RCF rmin 5 9 cm nmax 13000 min 1 RCF rmax nmax 12846 RCF speed min 1...

Страница 70: ...Appendix 66 1 10 100 1000 10000 100000 100 1000 10000 100000 75007595 drum rotor 80 x 2 ml RCF speed min 1 RCF rmax 8 7 cm RCF rmin 3 8 cm nmax 13000 min 1 RCF rmax nmax 16436...

Страница 71: ...Appendix 67 Autoclaving protocol Date Remark Operator Signature 1 2 3 4 5 6 7 8 9 10...

Страница 72: ...Appendix 68...

Страница 73: ...otors 28 autoclaving 43 autoclaving cycle permissible maximum 43 autoclaving protocol 67 B Beladungsvorschrift f r Rotor 7590 und 7595 22 Braking curves 59 braking profiles 9 32 buckets swinging bucke...

Страница 74: ...lays brief failure 47 not lit 47 drum rotor 16 racks 20 E Easycontrol user interface 9 Easycontrol user interface 11 Electrical connections 56 electronic unbalance detection 7 emergency lid release 45...

Страница 75: ...48 lid open during run warning 7 lid opening 27 lid unlocking mechanism manual 45 loading for aerosol tight operation 24 location 13 M mains connection 14 maintenance 39 manual lid unlocking 45 manual...

Страница 76: ...ion 36 quick run mode 9 R radius of centrifugation for calculation of RCF value 33 RCF maximum 35 RCF speed diagrams 61 RCF display and rotor identification 33 RCF setpoint 33 RCF value 9 17 33 RCF sp...

Страница 77: ...during run 35 short time operation 36 socket wrench 9 software check internal 27 software version determination 54 speed maximum 35 permissible 30 speed RCF diagrams 61 speed of centrifugation for cal...

Страница 78: ...inserting rotor 28 toxins protection against 4 transport precautions for 13 tube breakage with infectious material 40 tubes for centrifugation 9 U unbalance 30 unbalance detection 35 user interface 11...

Страница 79: ...s International Sales Asheville NC USA Tel 1 828 658 2711 Fax 1 828 645 9466 info kendro spx com North America Kendro Laboratory Products Asheville NC USA Tel 1 800 879 7767 Fax 1 828 658 0363 info ke...

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