44
12
Maintenance
Sterilizing the Dish Inserts
Use this procedure in cases of contamination and/or spillage:
Packaging:
1.
Promptly soak up excess liquid using a sterile cloth.
2.
Remove the Dish Insert from the incubator chamber.
3.
Moisten a sterile cloth with sterile water and wipe all surfaces, especially all of the milled grooves.
4.
Wrap Dish Inserts in a breathable sterilization wrap or place in a sterilization pouch in
accordance with local procedures. In the United States (US), use an FDA-cleared sterilization
wrap or sterilization pouch.
Steam Sterilization:
5.
Use a validated, properly maintained and calibrated steam sterilizer.
6.
Effective steam sterilization can be achieved using the following cycle:
Cycle Type Minimum Temperature Minimum Exposure Time / Dry Time
Gravity
121 °C (250 °F)
30 minutes / 30 minutes dry time
For further guidance for Sterilization, refer to: ANSI/AAMI ST79 “Comprehensive guide to steam
sterilization and sterility assurance in health care facilities.”
Validation Check
Perform the following validation gas and temperature checks after all cleaning, disinfecting
and sterilizing or at least every two weeks to ensure the G210 InviCell is operating correctly.
Gas Calibration
It is very important the the G210 is not emptied of gas during the gas calibration procedure. To do
so will cause unstable gas levels and gas flow and will result in a considerable time before the gas
concentration is recovered and becomes reliable and stable again.
To ensure the G210 is not emptied of gas during the gas sampling please follow the below instructions.
There are two methods for collecting gas samples from the incubator.
1.
Via the Gas Sample Port (located behind the Preparation Chamber)
2.
Via the silicone plug on each of the chamber lids
Always check the gas concentration in the setpoint menu during sampling. If the gas concentration
differs more than 0.1% from the setpoint, allow the G210 to stabilize to the gas concentration set
point before taking the next gas sample.
Allow 2 minutes between samples to allow the incubator to stabilise.
Please Note: To ensure accurate and reliable gas measurements, please use a high-quality calibrated
gas analyser. The calibration procedure described here assumes the use of the G100 gas analyser
(Order Code: 11103) which recirculates gas into the gas flow during sampling.
Ensure the gas analyzer is preperated as per the gas analyzer user maual.
Gas flow to the Preparation Chamber should be switched off during gas calibration. (See page 29,
Changing the Preparation Chamber Settings).
