iota Sciences isoCell, Руководство пользователя

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N.B. The heater is not intended to hold the dish at precisely 37°C. Rather its
purpose is to slow cooling when the dish is removed from an incubator.
When required by the workflow, user-supplied reagents must be placed in 1.5ml
microcentrifuge tubes (supplied in the CloneG consumables kit) at six defined
locations on the bed (
Figure 2 ). A minimum of 1ml of each required reagent should be
provided. Note that:
The isoCell will provide a visual prompt of the reagents required by any given
part of the workflow.
A seventh reagent, FC40
STAR
, is stored in the FC40
STAR
bottle at the rear of the
isoCell ( Figure 1 ).
Before beginning any program, ensure all microcentrifuge tubes are open
with their lids tucked under the rails, as shown in
Figure 2 . This is essential to
prevent obstruction of the needle holder.
Two further (empty) microcentrifuge tubes serve as repositories for aqueous and
FC40
STAR
waste. The isoCell will provide a visual prompt when waste microcentrifuge
tubes are required. Users should ensure that waste tubes are empty.
For instructions on the safe disposal of FC40
STAR
, refer to
Disposal of FC40
STAR
.
Cells are harvested by the isoCell from the 60mm dish into the two 8-tube PCR strips
(or flat bottom 8-well strips) located at the front of the bed (
Figure 2 ). The isoCell will
provide a visual prompt when the PCR or flat bottom strips are required. The strips
should be prepared with 120µl cell culture medium in each tube/well.
Do not put caps on the PCR strips.
Running programs
The isoCell workflow of isolating, culturing and harvesting cells is executed by
running a series of programs on the isoCell. For instance, the
GRID program will
create a GRID on the 60mm dish, the plate program will dispense cells into a GRID,
etc. All programs are executed from the isoCell’s interactive touchscreen. A stylus is
supplied for higher touch accuracy when required.
At the beginning of each program, a message on the touchscreen will prompt the
user to place the correct materials and reagents onto the
isoCell bed . When
providing reagents in microcentrifuge tubes, the user should fill the tube to a