iota Sciences isoCell, Руководство пользователя

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2.
Select colour. The user needs to select the colour of the locating ring attached to
their dish.
3.
Select chambers. The user needs to select coordinates of all chambers
containing a single cell.
4. Press the button to save data.
The isoCell supports up to thirty-six users. Under each username the isoCell
can simultaneously track up to nine individual dishes via the dishes’ coloured
locating rings. Stored user data can be managed via the
data program.
Culture
Two programs are provided for the culturing of single cells into monoclonal colonies:
fill
and
feed
.
Fill
The fill program is used to top up each chamber containing a single cell from 200nl
to 800nl. This must be the first program that is run on the dish following the input
data program.
Feed
The
feed program is used to remove 800nl waste from each monoclonal chamber,
and replace the removed fluid with 800nl fresh media. The user may run this
program as often as they deem suitable.
The
feed program should not be run on a dish containing suspension cells –
this would result in the cells being removed along with the 800nl waste.
Harvest
Two programs are provided for the harvesting of colonies:
detach
and
extract
. The
user can select up to sixteen colonies to harvest per run of the
detach and extract
programs.
Detach
The detach program is used to deliver dissociation reagent to the selected
chambers. It will first wash the chambers twice with PBS, after which it will dispense
800nl of dissociation reagent to each chamber.