iota Sciences isoCell, Руководство пользователя

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The
recover program (more
→
recover) is used to attempt to recover any chambers
from an
unknown state to a single-cell filled state (see chamber states
).
The program will operate on all chambers whose state is unknown. It will remove
800nl fluid from each chamber, and replace the removed fluid with 800nl fresh
media.
The
recover program is not guaranteed to successfully return colonies to a
useable state. If (for instance) chambers were left in an
unknown state
following an abort of the
detach
or
extract
programs, the colony may be
physically detached from the dish. In which case, the removal stage of the
recover program will likely remove and discard of the colony.
Calibrate
The
calibrate program (more
→
calibrate) is used to calibrate the dispensing and
jetting needle positions relative to the centre of the isoCell bed. It can be used if
either of the isoCell’s needles has been replaced, to ensure continued performance
of the isoCell.
Needle calibration happens automatically during the startup and restart
routines, and when replacing the dispensing assembly using the replace
dispensing
program.
Move
The move program (more → move) is used to:
Manually move the isoCell’s needle holder using the six arrow buttons indicated
in
Figure 3a . To return the needle holder to its home (not in use) position, press
the reset button.
Manually withdraw or insert the plungers of the dispensing and jetting syringes,
using the four buttons indicated in
Figure 3b .
The movement step size is set using the three buttons indicated in Figure 3b . Step
sizes are in millimetres for the needle holder and microlitres for the syringes. With the
step size set to 1.00 the move buttons will cause large/fast movements, whereas with
it set to 0.01 the move buttons will cause small/slow movements.