Invitrogen iBlot 2, Руководство пользователя

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15. Take the Top Stack from the bench and place it on top of the presoaked filter
paper with the copper electrode facing up (and transfer gel layer facing down).
Remove any air-bubbles using the Blotting Roller.
16. Place the iBlot ™ 2 Absorbent Pad on top of the iBlot ™ 2 Transfer Stack such that
the electrical contacts are aligned with the corresponding electrical contacts on
the blotting surface of the iBlot ™ 2 Gel Transfer Device.
17.
Use the Blotting Roller to flatten any protrusions in the transfer stack.
After assembling the iBlot
™
2 Gel Transfer Stack, perform blotting as described below.
Perform blotting within 10–15 minutes of assembling the stacks with the gel.
1. Gently close the iBlot ™ 2 Gel Transfer Device lid by pressing down with two
hands on the sides of the lid. Make sure the latch is secure. Do not forcibly push
the lid when closing, because it can cause the transfer stack or metal contacts to
shift out of position
2. Ensure that the correct Method is selected (“Recommended running
parameters“ on page 23).
3. Touch the Start icon on the screen to begin the transfer.
4. At the end of the transfer, the current automatically shuts off and the iBlot ™ 2 Gel
Transfer Device signals the end of transfer with repeated beeping sounds, and a
message on the digital display.
Performing
blotting
Chapter 2 Protein transfer protocol
Using the iBlot
™
2 Gel Transfer Device
2
iBlot
™
2 Dry Blotting System User Guide 33