Invitrogen iBlot 2, Руководство пользователя

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Schematic of iBlot ™ 2 Transfer Stack showing the flow of current
After the stack is assembled on the iBlot
™
2 Gel Transfer Device, and the appropriate
Method is selected, the run is initiated. Complete transfer of proteins from the gel to
the blotting membrane is accomplished in approximately 7–8 minutes. The rapid
transfer without the need for external power supply or premade buffers is possible
due to the following features of the iBlot
™
2 Dry Blotting System:
• The gel matrix of the Bottom and Top Stack incorporate the appropriate anode
and cathode buffers to act as ion reservoirs. This format eliminates the need for
premade buffers or soaked filter paper, and minimizes handling that can lead to
inconsistent performance.
• The copper anode does not generate oxygen gas as a result of water electrolysis,
resulting in increased transfer consistency. Conventional inert electrodes present
in other blotting systems result in oxygen generation, which can result in blotting
distortion.
• The design of the iBlot ™ 2 Gel Transfer Device reduces the distance between the
electrodes and the integrated power supply. This unique design combined with
the gel matrix technology of iBlot ™ 2 Transfer Stacks allows the system to
generate high field strength and increase the transfer speed.
Chapter 1 Product information
About the system
1
iBlot
™
2 Dry Blotting System User Guide 11