Invitrogen iBlot 2, Руководство пользователя

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Protein transfer protocol
Experimental overview
The table below outlines the experimental steps necessary to perform western blotting
using the iBlot ™ 2 Gel Transfer Device. For more details on each step, see indicated
pages.
Step Action Page
1
Select Method for
performing transfer.
“Selecting a method“ on
page 27
2
Remove the gel from the gel
cassette.
“Removing the gel“ on
page 28
3
Assemble the iBlot ™ 2
Transfer Stack with your
protein gel for transfer
using the iBlot ™ 2 Gel
Transfer Device.
“Assembling the iBlot ™ 2
Transfer Stack“ on page 29
4
Perform protein transfer
using the selected Method.
“Performing blotting“ on
page 33
5
Disassemble the iBlot
™
2
Transfer Stack.
“Disassembling the iBlot
™
2
Transfer Stack“ on page 34
General guidelines
To obtain the best results, follow these recommendations:
• Wear gloves at all times during the entire blotting procedure to prevent
contamination of gels and membranes.
• Do not touch the membrane or gel with bare or gloved hands. This may
contaminate the gel or membrane and interfere with further analysis. If you need
to adjust the membrane, always use forceps.
• Avoid using expired iBlot ™ 2 Transfer Stacks. Always use the transfer stacks
before the specified expiration date printed on the package.
• Remove air bubbles as indicated in the protocol using the Blotting Roller supplied
with the device.
2
Experimental
outline
iBlot
™
2 Dry Blotting System User Guide 25