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VaxArray® Imaging and Analysis System Operation Manual
CF-0159 R004
slope cutoff are set in the Analysis Variables menu as criteria for the linear fit to the calibration
curve.
D.
OFF:
Occasionally the term OFF (offscale) will be listed in the results. OFF indicates that the signal
of the corresponding sample is higher than the maximal signal of the corresponding standards. In
other words, the sample signal exceeds the upper limit of the standards.
6. Results Worksheet
To export a worksheet of results, click on the Excel icon in the bottom bar highlighted in
Figure 15
. This will
automatically open an Excel file with all raw data, calculations, and final concentrations.
A.
Excel Results in Raw Analysis Mode:
Raw Analysis Mode reports the RFU signals measured by the
instrument for the different antibodies.
These are reported in a table of results titled “Sample Signal
Values, Relative Fluorescent Units (RFUs)”.
In addition to RFUs, a corresponding table of results is
presented that normalizes the RFU signals by the maximum fluorescence value (65535), then
multiplies by the dilution factor associated with each sample. Dilution factors are entered when
setting up the experiment. This table is
referred to as “
Normalized Signals (Dilution Factor
Adjusted)
”
and is selectable in the results view via a toggle button. The table of raw and normalized
values is included in both the Excel file and the pdf report described below.
7. Generating a Report
To generate a formatted report of the experiment, click on the Report icon (highlighted in
Figure 16
) on the
bottom bar. This will automatically open the report as a pdf file.
Figure 15: Export Results.
