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VaxArray® Imaging and Analysis System Operation Manual
CF-0159 R004
Mismatched information entry will also be indicated by a red outline. For example,
dilution factors are restricted to positive real numbers. Entering text or negative numbers
will be indicated by a red outline until a positive number is entered. Other numeric
entries, like standard concentrations, have similar restrictions.
E.
Enter Standard Concentrations
: The table at the bottom of the Setup Experiment view records the
concentrations of the protein standards (
μ
g/mL) used for the calibration curve. During the VaxArray
assay procedure, these standards are processed in Column 1 of the slide. The user must enter
concentrations that match their specific experiment. Concentrations can be entered using several
different methods. The user can manually enter concentrations into every cell. Alternatively, if the
InDevR recommended dilution series was used during standard preparation, the user can manually
enter the highest concentrations, and the autopopulate feature of the table automatically fills in the
remaining standard concentrations. For example, entering a concentration value into Std 1 (High)
cell of H1(i) autofills the same concentration in all H1 Std 1 (High) cells. In addition, the lower
concentrations are autofilled following the fractional dilution series recommended by InDevR (0.8,
0.6, 0.4, 0.2, 0.1, and 0.05 multipliers). Any value can be overwritten by the operator. Another
option is to use a Standards Template. Once the appropriate standard concentrations are added to
the table, choose Create New and name the standards template for use in subsequent experiments.
a.
When operating in Raw Analysis Mode, the table is grayed out to indicate that raw analysis
does not apply a standard curve to determine protein concentrations. Raw Analysis Mode
provides the measured fluorescence signals of each antibody.
F.
Once all relevant information has been entered
: select the Right Arrow to proceed to Preview &
Image.
3. Preview & Image
The Preview & Image screen allows the user to preview an array in order to set the optimal exposure time as
well as to initiate an imaging event while providing real time array images (
).
Figure 10: Preview & Image.
