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VaxArray® Imaging and Analysis System Operation Manual
CF-0159 R004
A.
Select Target Antibodies
: The antibodies on the array layout image may be selected or deselected
to show/hide the calibration curves of specific antibodies. In general, antibodies with little or no
dynamic range may be hidden as the samples will not be able to be quantitated against these
antibodies.
a.
The vertical axis in the graphs is scaled based on the maximum signal for all antibodies of the
same type. For example, the vertical axis scale in Figure 11 has the same scale for the H1
antibodies and a different scale for the H3, B/Y, and B/V antibody types.
b.
Hovering the mouse pointer over the graphs will allow the user to see the signal value (RFU)
for each individual data point.
c.
The signals corresponding to the quantitation limit (QL) for each antibody are shown in a table
at the bottom left.
B.
Standards and Samples
: The calibration curves plot the signals from the standards as black
crosses/plus signs, and the signals from the samples are superimposed on the calibration curves as
colored circles.
When sample signals are near the quantitation limit or
on a “
plateau
”
region, users should
repeat the assay with modified dilution factors to place the signals closer to the middle of
the calibration curve.
C.
Select the Right Arrow to proceed to the Results Summary screen.
5. Review Results Summary
The Results Summary view provides a table of results for each sample and array antibody (
). Standard
analysis will provide results in
μ
g/mL units while Raw Analysis Mode will provide RFU (relative fluorescence unit)
values.
Figure 13: Calibration Curves.
