NanoPhotometer
®
N120/
NP80/N60/N50/C40
User Manual Version 4.3.1
50
4. Ensure that samples are mixed well to achieve homogenous samples.
5. Raise the lid arm and pipette 2 µl of blank solution onto the illuminated sample spots on
pedestal. Only illuminated sample windows will be measured. The Illumination turns off
automatically when the lid arm is lowered.
Note:
It is recommended to use multi-channel pipettes to avoid evaporation.
Note:
The low energy red light (LED) illumination can be switched off in Preferences.
6. Lower the lid arm and initiate a blank measurement with the blank button
7. Clean the measurement spots and mirrors in the lid arm with a slightly wet lint-free
tissue. Use water, 70% ethanol or isopropanol if needed.
Note:
Make sure that the metal contact face (around the measurement window and the
mirror) is clean.
Note:
Do not use aggressive solvents such as strong acids or bases or organic solvents
at any time (see page 55 solvent compatibility). If unsure please contact
for detailed information about your specific reagent/solvent.
8. Raise the lid arm and pipette 2µl of sample solution onto the illuminated sample
windows. Upon completion of measurement raise lid arm, clean the surfaces and apply
the next samples.
Note
: The sample window on pedestal must be clean and residual fluff from any
cleaning wipe must be removed for optimum performance.
