NanoPhotometer
®
N120/
NP80/N60/N50/C40
User Manual Version 4.3.1
22
N
ANO
P
HOTOMETER
®
S
PECIFICATIONS
NanoVolume Performance
Detection Range dsDNA
N60, NP80: 1 - 16,500 ng/µl
N50: 5 - 7,500 ng/µl
N120: 2 - 8,000 ng/µl
Detection Range BSA
N60, NP80: 0.03 - 478 mg/ml
N50: 0.15 - 217 mg/ml
N120: 0.06 - 230 mg/ml
Sample Volume
N50, N60, NP80: 0.3 - 2 µl
N120: 2 - 3.5 µl
Photometric Range
(10 mm equivalent)
N60, NP80: 0.02 - 330 A
N50: 0.1 - 150 A
N120: 0.04 - 160 A
Path length
N50, N60, NP80: 0.67 and 0.07 mm
N120: 1 and 0.125 mm
Dilution Factor
N50, N60, NP80: 15 and 140
N120: 10 and 80
Vortex
N60, NP80: 2,800 rpm; tube size up to 2.0 ml
Cuvette Performance
– NP80 & C40
Detection Range dsDNA
0.1 - 130 ng/µl
Detection Range BSA
0.003 - 3.7 mg/ml
Photometric Range
0 - 2.6 A
Center Height (Z-Height)
8.5 mm
Cell Types
outside dimension 12.5 x 12.5 mm
Heating
37°C ± 0.5°C
Optical Specifications
Wavelength Scan Range
C40, N60, NP80, N120: 200
– 900 nm
N50: 200 - 650 nm
Measure Time For Full Scan
Range
C40, N50, N60, NP80: 2.5
– 4.0 seconds
N120: 1.7
– 2.5 seconds per sample
Wavelength Reproducibility
C40, N60, NP80, N120: ± 0.2 nm
N50: ± 1nm
Wavelength Accuracy
C40, N60, NP80, N120: ± 0.75 nm
N50: ± 1.5 nm
Bandwidth
C40, N60, NP80: 1.8 nm
N50: 5 nm
N120: < 2.5 nm
Absorbance Reproducibility
C40, NP80 (cuvette): < 0.002 A @ 0 - 0.3 A @ 280 nm
CV < 1% @ 0.3
– 2.0 A @ 280 nm
N60, NP80 (Lid 15): < 0.002 A @ 0
– 0.3 A @ 280 nm
CV < 1% @ 0.3
– 1.7 A @ 280 nm
