Idexx SNAPshot Dx, Руководство оператора

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C-9
SNAP* Bile Acids
Test Description
An enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of bile acids in
canine and feline patients. The SNAP Bile Acids Test may be used as an initial screen to establish
a baseline level for hepatic function or as a bile acids stimulation test where standard protocols are
adhered to. Abnormal results obtained from the SNAP Bile Acids Test indicate hepatic dysfunction
ranging from mild, as with nonspecific hepatopathy, to severe, as with portosystemic shunts. Bile
acids may also be useful for monitoring the effects of therapeutic drugs on hepatic function and
screening for occult liver dysfunction when enzyme levels are normal.
Preparing and Running a SNAP Bile Acids Test
Important : Allow the SNAP Bile Acids Test and conjugate solution to equilibrate at room
temperature for at least 30 minutes. Do not heat. Keep the SNAP test in its sealed foil package until
ready to use.
1. Enter the patient information on the IDEXX VetLab Station (for more information, see the
“Analyzing Samples” chapter of the IDEXX VetLab Station Operator’s Guide ).
2. Once the patient’s name appears in the Pending list on the SNAPshot Dx Home screen, tap
the patient’s name and then tap Select . The Identify Sample screen appears.
3. Select Bile Acids as the SNAP Test Type.
4. Select the applicable sample type.
5. Tap
Next .
6. In the Protocol drop-down list, tap Preprandial , Postprandial , or Other .
7. Tap Next and follow the on-screen instructions to properly prepare the sample.
a. Use the gray handheld pipette (provided in the SNAPshot Dx sample prep kit) to transfer
100 μL of sample into a disposable sample tube.
b. Use the 300-μL pipette to transfer 300 μL of conjugate into the same sample tube.
c. Cap the sample tube and gently invert it 4–5 times to mix.
8. Tap Next and follow the on-screen instructions to properly prepare the SNAP test:
a. Place the device on a horizontal surface.
b. Add the entire contents of the sample tube to the sample well, being careful not to
splash the contents outside of the sample well. The sample will flow across the result
window, reaching the activation circle in 30–60 seconds. Some sample may remain in
the sample well.
c. When color FIRST appears in the activation circle, press the activator firmly until it is
flush with the device body.
9. Insert the SNAP device into the SNAPshot Dx Analyzer immediately. The SNAPshot Dx
Analyzer automatically begins to process the patient sample and the Home screen displays.
The patient’s name moves from the Pending list to the In Process list.
When analysis is complete, the patient’s name will move from the In Process list to the Results
list (you may also receive a “New Results” alert message and/or hear a beep on the IDEXX
VetLab Station, depending on the option(s) you selected in the New Results Alert tab on the
IDEXX VetLab Station’s Settings screen). You can then remove the SNAP device from the
analyzer by gently pushing the SNAP device until you hear a click. The device will slide out of
the port.
SNAP Test Procedures