C-6
Protocol/Protocol Step
Sample
Volume
Pipette
Color
Conjugate
Volume
Range
†
ACTH Stim Cortisol: Therapeutic
Monitoring
100 µL
Gray
300 µL
0.5–10.0 µg/dL
(13.8–276.0
nmol/L)
ACTH Stim Cortisol: Therapeutic
Monitoring >10 µg/dL
25 µL
Blue
300 µL
2.5–30.0 µg/dL
(69.0–828.0
nmol/L)
†
“Range” refers to the test result range for a particular test’s protocol. When a test result falls outside of this
range, it will be reported as either “>” (greater than the upper limit) or “<” (less than the lower limit).
b. Cap the sample tube and gently invert it 4–5 times to mix.
c. Place the sample tube into an available incubator well.
d. Tap to start the incubation timer that corresponds to the well you selected (left or right).
The incubation timer displays with the patient information in the Pending list on the Home
screen (the timer counts down in 10-second intervals).
8. When incubation is complete (the patient information in the Pending list changes from
“Incubating” to “Ready,” the text turns red, and the analyzer beeps), tap to select the patient in
the Pending list.
9. Tap
Next
and follow the on-screen instructions to properly prepare the SNAP test:
a. Place the device on a horizontal surface.
b. Add the entire contents of the sample tube to the sample well, being careful not to
splash the contents outside of the sample well. The sample will flow across the result
window, reaching the activation circle in 30–60 seconds. Some sample may remain in
the sample well.
c. When color FIRST appears in the activation circle, press the activator firmly until it is
flush with the device body.
10. Insert the SNAP device into the SNAPshot Dx Analyzer immediately. The SNAPshot Dx
Analyzer automatically begins to process the patient sample and the Home screen displays.
The patient’s name moves from the Pending list to the In Process list.
When analysis is complete, the patient’s name will move from the In Process list to the Results
list (you may also receive a “New Results” alert message and/or hear a beep on the IDEXX
VetLab Station, depending on the option(s) you selected in the New Results Alert tab on the
IDEXX VetLab Station’s Settings screen). You can then remove the SNAP device from the
analyzer by gently pushing the SNAP device until you hear a click. The device will slide out of
the port.
Interpreting Test Results
The recommendations below are intended to provide general guidance only. In all cases, it is
important to exercise clinical judgement in interpreting cortisol results in the context of the patient’s
history, clinical signs, physical status, and other laboratory data.
Note:
Dexamethasone (doses >0.1 mg/kg), prednisone, and other corticosteroids are expected to
artificially increase the reported result.
SNAP Test Procedures
Содержание SNAPshot Dx
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