Idexx SNAPshot Dx, Руководство оператора

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C-5
SNAP* Cortisol
Test Description
An enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of cortisol in
canine patients. With the SNAP Cortisol Test (when used in conjunction with accepted protocols
for ACTH stimulation and low-dose dexamethasone suppression tests), you can screen for
Addison’s disease and Cushing’s syndrome. You can also monitor response to treatment and make
adjustments to medications for patients diagnosed with Cushing’s syndrome.
Important : DO NOT use the SNAP Cortisol Test if the patient has received:
• Dexamethasone (doses >0.1 mg/kg) in the last 7 days.
OR
• Prednisone or other corticosteriods in the past 4 weeks.
Preparing and Running a SNAP Cortisol Test
Important : Allow the SNAP Cortisol Test to equilibrate in its sealed foil package at room temperature
for at least 10 minutes. Do not heat. It is not necessary to equilibrate the conjugate solution at room
temperature.
1. Enter the patient information on the IDEXX VetLab Station (for more information, see the
“Analyzing Samples” chapter of the IDEXX VetLab Station Operator’s Guide ).
2. Once the patient’s name appears in the Pending list on the SNAPshot Dx Home screen, tap the
patient’s name and then tap Select . The Identify Sample screen appears.
3. Select Cortisol as the SNAP Test Type (the sample type is chosen automatically).
4. Tap Next .
5. In the Protocol drop-down list, tap ACTH Stim , LDDST , or HDDST .
Note: Take care not to exceed 0.1 mg/kg of dexamethasone.
6. In the Protocol Step drop-down list, tap the applicable protocol step. The options that are
available vary based on the Protocol option that was selected in step 6.
7. Tap Next and follow the on-screen instructions to properly prepare the sample.
a. Using the appropriate handheld pipette (provided in the SNAPshot Dx sample prep kit),
transfer the sample and conjugate into a disposable sample tube (see volumes below).
Protocol/Protocol Step Sample
Volume Pipette
Color Conjugate
Volume
Range
†
Baseline Cortisol
100 µL Gray 300 µL
0.5–10.0 µg/dL
(13.8–276.0
nmol/L)
Dexamethasone Suppression
Cortisol (low- or high-dose)
100 µL Gray 300 µL
0.5–10.0 µg/dL
(13.8–276.0
nmol/L)
ACTH Stim Cortisol: Cushing’s
Suspected
25 µL Blue 300 µL
2.5–30.0 µg/dL
(69.0–828.0
nmol/L)
ACTH Stim Cortisol: Addison’s
Suspected
100 µL Gray 300 µL
0.5–10.0 µg/dL
(13.8–276.0
nmol/L)
SNAP Test Procedures