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c. Using light downward pressure, slowly and steadily fill the cartridge window to avoid air
bubbles. If using the pipette, only depress plunger to the first stop.
d. Ensure the sample fills the cartridge window but not the overflow channel. If the
cartridge is overfilled, complete the cleaning procedure on page 24
before
pressing
the Start button.
e. Lift the pipette tip from the fill port before you release the plunger.
4. Press the
Start
button on the front of the analyzer. A progress bar displays next to the icon
in the patient’s job status area on the IDEXX VetLab Station Home screen, indicating the
percentage of the run that has completed.
Diluting samples
When urine samples are crowded with cells, bacteria, debris, or crystals, it may be helpful to dilute
the sample to spread the elements apart for easier identification and classification. To determine
whether a preanalysis or postanalysis dilution is recommended, see the “Dilutions” section on the
next page.
1. Initiate the sample run on the IDEXX VetLab
Station.
OR
If the patient has already been run once, select
the patient from the Recent Test Results list, tap
Add Test
,
and then tap the SediVue Dx icon.
2. Tap
Run Dilution
.
3. Use the up/down arrows to specify the desired
dilution factor (total parts).
4. Tap
Run
or
Append Results
.
5. In a test tube, dispense 165 μL of the well-mixed
urine sample. Add the selected parts of 0.9%
normal saline and invert the sample 10 times to
mix.
6.
IMPORTANT:
Aspirate 165 μL from the bottom third of the diluted sample and then
immediately inject it into the cartridge fill port.
7. Press the
Start
button on the analyzer. The results will be adjusted by the dilution factor and
noted that the sample was diluted.
If the dilution does not yield semiquantitative results, repeat the process using a higher
dilution factor.
Note:
Diluting a urine sample may affect the pH and osmolality of the sample and lead to changes
in cellular appearance and the presence of crystals.
Running a Sample on the Analyzer
Enter the dilution information in the center of
the Select Instruments screen.
Содержание SediVue Dx
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