12
Preparing samples
Urine samples that are run on the SediVue Dx* analyzer can be obtained
via cystocentesis, catheterization, or free catch method. For best results,
analyze urine samples within
30 minutes
of collection. Otherwise, store
the sample in the refrigerator for up to 2 hours.
Note:
Storing urine samples in the refrigerator may introduce the
in vitro
formation of crystals and crystalline debris.
If the sample is in a syringe:
1. Ensure there is at least 0.5 mL of sample in a 1 mL–5 mL syringe.
2. Draw 0.5 mL of air into the syringe.
3. Replace the needle with a SediVue* syringe tip.
4. Gently invert the syringe 10 times to mix the sample.
5. Immediately after inversion, purge all of the air from the syringe and inject 165 μL of urine into
the cartridge fill port.
If the sample is in a container:
1. Cap the container and invert it 10 times to mix—a well-mixed sample is essential to obtaining
accurate results.
2. Immediately after inversion, use the SediVue* Pipette to aspirate
165 μL of sample from the bottom third of the container, ensuring
there are no bubbles in the sample, and immediately inject 165 μL
of urine into the cartridge fill port.
IMPORTANT:
Crystals start to settle immediately after inversion.
Be sure to aspirate the sample from the bottom third of the
sample. Then remove excess urine on the outside of the tip with a
lint-free wipe.
Analyzing a sample
1. Initiate the sample run on the IDEXX VetLab Station (for more
information, see the
IDEXX VetLab Station Operator’s Guide
).
2. After you’ve confirmed the patient information on the Select
Instruments screen, select the
SediVue Dx
icon, select the fluid
type if it is not already listed, and then tap
Run
.
3. Once the analyzer has placed a cartridge in the pipetting window,
invert the sample 10 times and then immediately inject 165 μL of
urine into the cartridge fill port using either the SediVue Pipette or
a syringe with a SediVue Syringe Tip attached.
IMPORTANT—When injecting sample into the cartridge:
a. Use only IDEXX-supplied pipette tips and syringe tips.
They are made specifically for the SediVue Dx analyzer
and designed to reduce the risk of spills, leaks, and
inappropriately filling the cartridge.
b. Hold the pipette or syringe vertically to ensure the sample is injected only into the
cartridge. Make sure the pipette/syringe tip is firmly seated into the fill port on the
cartridge.
Running a Sample on the Analyzer
3
4
2
Stop
3
Содержание SediVue Dx
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