Hoefer SE 260 Скачать руководство пользователя страница 24 | Manualshive

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Hoefer SE 260 Скачать руководство пользователя страница 24

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p20

Final concentrations

separating gel

stacking gel

electrophoresis

buffer

Acrylamide conc.

10% T*, 2.6% C

4% T, 2.6% C

Tris-Cl

0.375 M

0.125 M

Tris-glycine

0.025 M Tris base

0.192 M glycine

pH

8.8

6.8

~8.3

SDS

0.1%

0.1%

0.1%

Ammonium persulfate (APS)

0.05% w/v

0.05–0.1% w/v

TEMED

†

0.05% v/v

0.05–0.1% v/v

* To achieve any other desired ﬁnal concentration, adjust the acrylamide stock and water volumes.
†Tetramethylethylenediamine

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Содержание SE 260

Страница 1: ...user manual SE 260 um SE260 IM Rev A0 05 04 Hoefer SE 260 mini vertical gel electrophoresis unit...

Страница 2: ......

Страница 3: ...Page nder 1 Gel Electrophoresis Unit Function 1 and Description 2 Important information 4 3 Operating instructions 6 4 Care and maintenance 16 5 Troubleshooting 17 Appendix Bibliography 21 pi...

Страница 4: ...pii...

Страница 5: ...aled against the silicone rubber gasket The upper buffer chamber core serves as a heat exchanger if cooling is required The core is hollow and equipped with ports on either side for coolant circulatio...

Страница 6: ...oducts recommended or approved by Hoefer Inc Speci cations Gel plate size 10 10 5 cm Approximate gel size 8 9 5 cm Max wattage 12 W Max voltage 500 V Max amperage 500 mA Max temperature 45 C Environme...

Страница 7: ...pring clamps 4 SE252 Coolant port 2 Foam gasket SE208 Fig 1 Main components SE 260 electrophoresis unit Included but not shown Glass plates Notched alumina plates Gel seal 1 4 oz Spacer Mate Well loca...

Страница 8: ...tap or any coolant source where the water pressure is unregulated Do not operate with buffer temperature above 45 C All plastic parts are rated for 45 C continuous duty Circulate coolant through the...

Страница 9: ...pression n est pas r guli re Ne pas utiliser avec un tampon une temp rature au dessus de 45 C Toutes les pi ces en plas tique sont pr vues pour r sister une temp rature constante de 45 C Faire circule...

Страница 10: ...using a notched alumina ceramic back plate because it transfers heat 40 times more rapidly than glass For applications that are not heat sensitive a notched glass plate is available Before loading gel...

Страница 11: ...etching the gasket by laying it onto the groove and pressing it into place Optional cooling Circulating pressure must not exceed 0 8 bar 12 psi above ambient pressure Do not connect the cooling core t...

Страница 12: ...to place Alternatively depress both release tabs at either side position the core on the positioning tabs press into place and release the tabs Check that the core is secure To remove the core depress...

Страница 13: ...alling a self cast or precast 10 10 5 cm gel sandwich orient the sandwich so that the notched plate faces the gasket notches at the top Set the bottom of the sandwich on the supporting ledges in the b...

Страница 14: ...or if running only one gel clamp a plain glass plate on the unused side of the core to prevent a possible short circuit with the unused electrode Do not ll this chamber with buffer if no gel sandwich...

Страница 15: ...comb sides to rest on the spacers Prepare the sample Increase liquid sample density with 10 glycerol or sucrose Add a tracking dye such as phenol red or bromophenol blue For SDS protein gels use 2X tr...

Страница 16: ...e sample wells and each upper buffer cham ber that will be used with running buffer One upper buffer chamber holds approximately 75 mL Underlay the sample into the wells using a ne tipped microsyringe...

Страница 17: ...urface is exposed the colored plastic tape must be removed Place the safety lid on the unit Plug the color coded leads into the jacks of an approved power supply such as the EPS 2A200 The red lead plu...

Страница 18: ...A lower current setting is recom mended for higher resolution Precast gels are run under the same current and voltage condi tions as self cast gels It takes about one hour to run two 7 cm 0 75 mm Laem...

Страница 19: ...verting the core assem bly then remove both clamps and lift away gel sandwich es from the upper buffer chamber core Gently loosen and then slide away both spacers Slip an extra spacer or a Hoefer Wond...

Страница 20: ...rinse the unit with water and then rinse thoroughly with distilled water Handle the upper buffer chamber core with care to prevent damage to the banana plugs Allow to air dry Clean glass and alumina p...

Страница 21: ...Tris HCl instead of Tris If the required pH of a solution is exceeded do not back titrate Prepare fresh buffer Dispose of older acrylamide solutions and use only stock of the highest quality Only use...

Страница 22: ...more mercaptoethanol or dithiothreitol check sample treatment Only use gels that were recently prepared Check pH values of the separating and stacking gel solutions Do not back titrate buffers Sample...

Страница 23: ...is indicated by two different percentages T total acrylamide g acryl bis 100 100 mL C crosslinker g bis 100 g acryl bis The total percent of acrylamide T in the separating gel which can range from 5...

Страница 24: ...T 2 6 C Tris Cl 0 375 M 0 125 M Tris glycine 0 025 M Tris base 0 192 M glycine pH 8 8 6 8 8 3 SDS 0 1 0 1 0 1 Ammonium persulfate APS 0 05 w v 0 05 0 1 w v TEMED 0 05 v v 0 05 0 1 v v To achieve any...

Страница 25: ...D R SDS microslab linear gradient polyacrylamide gel electrophoresis Anal Biochem 87 386 396 1978 Reisfeld R A et al Acidic buffer system for resolu tion of cationic proteins Nature 195 281 1962 Sasse...

Страница 26: ...p22...

Страница 27: ...sis Unit Replacement Parts Foam gasket 4 5 mm 61 cm 1 SE208 Upper buffer chamber for SE 260 1 SE254B Deep lower buffer chamber for SE 260 1 SE255D Lid with cables for SE 260 1 SE256 Wonder Wedge plate...

Страница 28: ...h mm 5 0 75 13 0 1 SE211A 5 75 5 1 00 13 0 1 SE211A 5 1 0 5 1 50 13 0 1 SE211A 5 1 5 9a 1 00 5 8 1 SE211A 9 1 0 10 0 75 4 8 1 SE211A 10 75 10 1 00 4 8 1 SE211A 10 1 0 10 1 50 4 8 1 SE211A 10 1 5 15 0...

Страница 29: ...ncludes 10 rectangular glass plates 4 notched alumina plates 100 sheets of wax paper space saver plate 5 ller sheets Spacer Mate and ller plugs Order combs and spacers separately For 2 to 4 gels 10 10...

Страница 30: ...p26...

Страница 31: ......

Страница 32: ...Hoefer Inc 953 Indiana Street San Francisco CA 94107 USA www hoeferinc com Printed in the USA...

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